DGCR8 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-30116
![Immunohistochemistry: DGCR8 Antibody [NBP1-30116]](https://resources.rndsystems.com/images/products/DGCR8-Antibody-Immunohistochemistry-NBP1-30116-img0007.jpg "Immunohistochemistry: DGCR8 Antibody [NBP1-30116]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antigen maps to a region between residue 400 and 450 of human DiGeorge syndrome critical region gene 8 using the numbering given in entry NP_073557.3 (GeneID 54487).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DGCR8 Antibody - BSA Free
Immunohistochemistry: DGCR8 Antibody [NBP1-30116]
Immunohistochemistry: DGCR8 Antibody [NBP1-30116] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DGCR8 used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB![Immunoprecipitation: DGCR8 Antibody [NBP1-30116]](https://resources.rndsystems.com/images/products/DGCR8-Antibody-Immunoprecipitation-NBP1-30116-img0006.jpg "Immunoprecipitation: DGCR8 Antibody [NBP1-30116]")
Immunoprecipitation: DGCR8 Antibody [NBP1-30116]
Immunoprecipitation: DGCR8 Antibody [NBP1-30116] - Whole cell lysate from HeLa cells. NBP1-30116 used for IP at 3 mcg/mg lysate. STK11IP was also IPed by rabbit STK11IP antibody NBP1-30115. For blotting IPed STK11IP, NBP1-30115 was used at 1 mcg/ml.Applications for DGCR8 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500-1:2000
Immunohistochemistry-Paraffin
1:500-1:2000
Immunoprecipitation
2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: DGCR8
Alternate Names
C22orf12, DGCRK6chromosome 22 open reading frame 12, DiGeorge syndrome critical region 8, DiGeorge syndrome critical region gene 8, Gy1, microprocessor complex subunit DGCR8, pasha
Entrez Gene IDs
54487 (Human)
Gene Symbol
DGCR8
UniProt
Additional DGCR8 Products
Product Documents for DGCR8 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DGCR8 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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