DGK-epsilon Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85315

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DGK-epsilon Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLRKADKRFQCKEIMLKNDTKVLDAMPHHWIRGNVPLCSYCMVCKQQCGCQPKLCDYRCIWCQKTVHDECMKNSLKNEKCDFGEFKNLIIPPSYL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DGK-epsilon Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DGK-epsilon Antibody [NBP1-85315]

Immunocytochemistry/ Immunofluorescence: DGK-epsilon Antibody [NBP1-85315]

Immunocytochemistry/Immunofluorescence: DGK-epsilon Antibody [NBP1-85315] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm.
Immunohistochemistry-Paraffin: DGK-epsilon Antibody [NBP1-85315]

Immunohistochemistry-Paraffin: DGK-epsilon Antibody [NBP1-85315]

Immunohistochemistry-Paraffin: DGK-epsilon Antibody [NBP1-85315] - Staining of human testis shows distinct positivity in spermatids and spermatozoa.

Applications for DGK-epsilon Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DGK-epsilon

Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.

Long Name

Diacylglycerol Kinase, epsilon 64kDa

Alternate Names

DAGK5, DAGK6, DGKE, DGKepsilon

Gene Symbol

DGKE

Additional DGK-epsilon Products

Product Documents for DGK-epsilon Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DGK-epsilon Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

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Protocols

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FAQs

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