DHPS Antibody - BSA Free

Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82648]

Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82648] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82648]

Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82648] - Staining of human fallopian tube shows moderate cytoplasmic/membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82648]

Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82648] - Staining of human pancreas shows weak cytoplasmic positivity in islets of Langerhans.

Western Blot: DHPS Antibody - BSA Free [NBP1-82648] -

Spermidine supplementation restored hepatic Eif5aH and mitochondrial protein levels in a dietary mouse model of NASH.a–c Western blot and densitometric analysis of hepatic Dhps, Dohh, Eif5aH, and Eif5a in NCD vs. WDF (a), and WDF vs. WDF + Spd (b) mice. The blots in a and b were processed in parallel. Densitometric analysis (c) was first normalized with GAPDH, and then calculated the fold change against WDF (NCD vs WDF, and WDF + Spd vs WDF). (n = 6) (d–f) Western blot and densitometric analysis of Tfam, PGC1 alpha, and mitochondrial proteins in the liver from mice fed with NCD vs. WDF (d), or WDF vs. WDF + Spd (e). The blots in d and e were processed in parallel. Densitometric analysis (f, n = 6) was first normalized with GAPDH, and then calculated the fold change against WDF (NCD vs WDF, and WDF + Spd vs WDF). (g, h) Mitochondrial DNA copy number (g) and circulating beta -hydroxybutyrate (h) in NCD (n = 6), WDF (n = 6), and WDF + Spd (n = 6) groups. Significance was calculated by one-way ANOVA or Kruskal–Wallis test, as appropriate. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36057633), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: DHPS Antibody - BSA Free [NBP1-82648] -

Spermidine supplementation restored hepatic Eif5aH and mitochondrial protein levels in a dietary mouse model of NASH.a–c Western blot and densitometric analysis of hepatic Dhps, Dohh, Eif5aH, and Eif5a in NCD vs. WDF (a), and WDF vs. WDF + Spd (b) mice. The blots in a and b were processed in parallel. Densitometric analysis (c) was first normalized with GAPDH, and then calculated the fold change against WDF (NCD vs WDF, and WDF + Spd vs WDF). (n = 6) (d–f) Western blot and densitometric analysis of Tfam, PGC1 alpha, and mitochondrial proteins in the liver from mice fed with NCD vs. WDF (d), or WDF vs. WDF + Spd (e). The blots in d and e were processed in parallel. Densitometric analysis (f, n = 6) was first normalized with GAPDH, and then calculated the fold change against WDF (NCD vs WDF, and WDF + Spd vs WDF). (g, h) Mitochondrial DNA copy number (g) and circulating beta -hydroxybutyrate (h) in NCD (n = 6), WDF (n = 6), and WDF + Spd (n = 6) groups. Significance was calculated by one-way ANOVA or Kruskal–Wallis test, as appropriate. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36057633), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: DHPS Antibody - BSA Free [NBP1-82648] -

Decreased DHPS-DOHH-EIF5AH pathway in NAFLD.a Polyamine synthesis and hypusination of EIF5A pathway in eukaryotic cells. Enzymes ARG1, ODC, SRM are involved in converting arginine to spermidine. DHPS and DOHH use spermidine to hypusinate EIF5A. b Violin plots showing mRNA levels of genes involved in endogenous polyamine biosynthesis and EIF5A hypusination in Control (n = 19), steatosis (n = 10), and NASH (n = 16) from publicly available database (accession number E-MEXP-3291, http://www.webcitation.org/5zyojNu7T)20. c Violin plots showing mRNA levels of genes involved in endogenous polyamine biosynthesis and EIF5A hypusination in Control (n = 12), steatosis (n = 9), and NASH (n = 17) from publicly available database (GSE48452)21. b, c Significance was calculated by one-way ANOVA or Kruskal–Wallis test, as appropriate. d Quantitative-PCR analysis of mRNA levels of polyamine metabolism genes in the livers from mice fed with NCD (n = 8) or WDF (n = 8) for 16 weeks. e Western blot and densitometric analysis of protein levels of Dhps, Dohh, eIF5AH, and eIF5A in the liver from mice fed with NCD (n = 7) or WDF (n = 6) for 16 weeks. f, g mRNA expression of genes in polyamine biosynthesis and hypusination pathways (f, n = 5), and protein levels of Dhps, Dohh, eIF5AH, and eIF5A (g, n = 3) in AML12 hepatic cells treated with fatty acids (FA, palmitic acid 0.6 mM, oleic acid 0.17 mM) for 48 h. f–g Data were shown as box-and-whisker with median (middle line), 25th–75th percentiles (box), and min-max values (whiskers). d–g significance was calculated by two-tailed Student’s t test or Mann–Whitney U test, as appropriate. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36057633), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: DHPS Antibody - BSA Free [NBP1-82648] -

Decreased DHPS-DOHH-EIF5AH pathway in NAFLD.a Polyamine synthesis and hypusination of EIF5A pathway in eukaryotic cells. Enzymes ARG1, ODC, SRM are involved in converting arginine to spermidine. DHPS and DOHH use spermidine to hypusinate EIF5A. b Violin plots showing mRNA levels of genes involved in endogenous polyamine biosynthesis and EIF5A hypusination in Control (n = 19), steatosis (n = 10), and NASH (n = 16) from publicly available database (accession number E-MEXP-3291, http://www.webcitation.org/5zyojNu7T)20. c Violin plots showing mRNA levels of genes involved in endogenous polyamine biosynthesis and EIF5A hypusination in Control (n = 12), steatosis (n = 9), and NASH (n = 17) from publicly available database (GSE48452)21. b, c Significance was calculated by one-way ANOVA or Kruskal–Wallis test, as appropriate. d Quantitative-PCR analysis of mRNA levels of polyamine metabolism genes in the livers from mice fed with NCD (n = 8) or WDF (n = 8) for 16 weeks. e Western blot and densitometric analysis of protein levels of Dhps, Dohh, eIF5AH, and eIF5A in the liver from mice fed with NCD (n = 7) or WDF (n = 6) for 16 weeks. f, g mRNA expression of genes in polyamine biosynthesis and hypusination pathways (f, n = 5), and protein levels of Dhps, Dohh, eIF5AH, and eIF5A (g, n = 3) in AML12 hepatic cells treated with fatty acids (FA, palmitic acid 0.6 mM, oleic acid 0.17 mM) for 48 h. f–g Data were shown as box-and-whisker with median (middle line), 25th–75th percentiles (box), and min-max values (whiskers). d–g significance was calculated by two-tailed Student’s t test or Mann–Whitney U test, as appropriate. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36057633), licensed under a CC-BY license. Not internally tested by Novus Biologicals.