DHX15 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-586

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DHX15 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 750 and the C-terminus (residue 795) of human DEAH box polypeptide 15 using the numbering given in Swiss-Prot entry O43143 (GeneID 1665).

Specificity

NB100-586 is specific for human DHX 15 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DHX15 Antibody - BSA Free

Western Blot: DHX15 Antibody [NB100-586]

Western Blot: DHX15 Antibody [NB100-586]

Western Blot: DHX15 Antibody [NB100-586] - Detection of Human DHX15 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX15 antibody NB100-586 used for WB at 0.066 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: DHX15 Antibody [NB100-586]

Western Blot: DHX15 Antibody [NB100-586]

Western Blot: DHX15 Antibody [NB100-586] - Detection of Human DHX15 on HeLa whole cell lysate using NB100-586. Immunoprecipitated DHX15 was blotted using NB100-585 at 0.1 mcg/ml. NB100-585 was also used unsuccessfully for IP.

Applications for DHX15 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1-5 ug/mg lysate

Western Blot

1:5000-1:15000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:5,000 - 1:15,000 Immunoprecipitation: 1 to 5 ug/mg lysate Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.

Reviewed Applications

Read 1 review rated 5 using NB100-586 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DHX15

The protein encoded by this gene is a putative ATP-dependent RNA helicase implicated in pre-mRNA splicing.

Alternate Names

ATP-dependent RNA helicase #46, DBP1DEAH box protein 15, DDX15putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 15, DEAH (Asp-Glu-Ala-His) box polypeptide 15, EC 3.6.1, EC 3.6.4.13, HRH2DEAD/H box-15, PRP43, PRPF43, PrPp43p, RNA helicase 2

Entrez Gene IDs

1665 (Human)

Gene Symbol

DHX15

UniProt

O43143 (Human)

Additional DHX15 Products

Product Documents for DHX15 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DHX15 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DHX15 Antibody - BSA Free

Customer Reviews for DHX15 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used DHX15 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • DHX15 Antibody - BSA Free
    Name: Anonymous
    Application: Western Blot
    Sample Tested: breast cancer MCF7 cells
    Species: Human
    Verified Customer | Posted 06/19/2025
    DHX15 protein levels in MCF7 cells
    DHX15 Antibody - BSA Free NB100-586

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies