DHX30 Antibody

Novus Biologicals | Catalog # NBP1-26603

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Primate (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all DHX30 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 350 and 400 of human DEAH (Asp-Glu-Ala-His) box polypeptide 30 using the numbering given in entry NP_619520.1 (GeneID 22907).

Reactivity Notes

Orangutan (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DHX30 Antibody

Western Blot: DHX30 Antibody [NBP1-26603]

Western Blot: DHX30 Antibody [NBP1-26603]

Western Blot: DHX30 Antibody [NBP1-26603] - Whole cell lysate from HeLa and 293T cells. DHX30 was also immunoprecipitated by rabbit anti-DHX30 antibody NBP1-26604.
Immunoprecipitation: DHX30 Antibody [NBP1-26603]

Immunoprecipitation: DHX30 Antibody [NBP1-26603]

Immunoprecipitation: DHX30 Antibody [NBP1-26603] - 5ug antibody used with 500ug B-cell lysate. Same antibody used for western blot 1:2000. 5 minute exposure. Image from verified customer review.

Applications for DHX30 Antibody

Application
Recommended Usage

Immunoprecipitation

5-10 ug/mg lysate

Western Blot

1:2000-1:10000

Reviewed Applications

Read 3 reviews rated 2.7 using NBP1-26603 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DHX30

DHX30 (DEAH-box polypeptide 30) is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DHX30 has been implicated to be involved in the packaging of HIV-1 viral RNA and HIV-1 replication.

Alternate Names

ATP-dependent RNA helicase DHX30, DDX30, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 30, DEAH (Asp-Glu-Ala-His) box polypeptide 30, DEAH box protein 30, EC 3.6.1, EC 3.6.4.13, FLJ11214, KIAA0890RETCOR, putative ATP-dependent RNA helicase DHX30, retina co-repressor

Entrez Gene IDs

22907 (Human)

Gene Symbol

DHX30

UniProt

NP_619520.1 (Human)

Additional DHX30 Products

Product Documents for DHX30 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DHX30 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for DHX30 Antibody (3)

2.7 out of 5
3 Customer Ratings
5 Stars
0%
4 Stars
33%
3 Stars
33%
2 Stars
0%
1 Stars
33%

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Customer Images

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  • DHX30 Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: B-cell lysate
    Species: Human
    Verified Customer | Posted 02/19/2019
    DHX30 immunoprecipitation in human B-cell lysate.
    5ug antibody used with 500ug B-cell lysate. Same antibody used for western blot 1:2000. 5 minute exposure
    DHX30 Antibody NBP1-26603
  • DHX30 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Lymphocytes
    Species: Human
    Verified Customer | Posted 03/13/2018
    1:2000 dilution in 5% milk TBS-T incubated overnight 4C. 3 minute exposure
    BIOSCARF
    DHX30 Antibody NBP1-26603
  • DHX30 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 01/11/2018
    1:100 in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C. Staining didn't show localization to mitochondria, looked nuclear/throughout cell. There are other validated antibodies for immunofluorescence. This antibody was purchased for western blot, and I wanted to test it for immunofluorescence.
    DHX30 Antibody NBP1-26603

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FAQs for DHX30 Antibody

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  • Q: Have you ever tried this antibody on immunohistochemistry on paraffin section?

    A:

    We have not yet tested this product for IHC-P staining. If it had been tested and confirmed it would be listed, and if it had been tested and confirmed not to work, or reported not to work by a customer, we would list the applications as IHC-P (-). If you would like to test this one in the new application and provide feedback on the results I can recommend our Innovators Reward Program.

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