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Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 150 and200 of human DEAH (Asp-Glu-Ala-Asp/His) box polypeptide 57 using the numbering given in entry NP_945314.1 (GeneID 90957).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DHX57 Antibody
Western Blot: DHX57 Antibody [NBP1-28738]
Western Blot: DHX57 Antibody [NBP1-28738] - Detection of Human DHX57 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Applications for DHX57 Antibody
Application
Recommended Usage
Immunoprecipitation
10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: DHX57
Alternate Names
DDX57, DEAH (Asp-Glu-Ala-Asp/His) box polypeptide 57, DEAH box protein 57, DEAH-box RNA/DNA helicase AAM73547, EC 3.6.1, EC 3.6.4.13, FLJ32861, putative ATP-dependent RNA helicase DHX57
Entrez Gene IDs
90957 (Human)
Gene Symbol
DHX57
UniProt
Additional DHX57 Products
Product Documents for DHX57 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DHX57 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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