DIAPH-2 Antibody (CL1111) - BSA Free

Novus Biologicals | Catalog # NBP2-52966

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1111

Format

BSA Free
View all DIAPH-2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from O60879, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for DIAPH-2 Antibody (CL1111) - BSA Free

Western Blot: DIAPH-2 Antibody (CL1111) [NBP2-52966]

Western Blot: DIAPH-2 Antibody (CL1111) [NBP2-52966]

Western Blot: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG
Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human uterus shows strong cytoplasmic immunoreactivity in glandular cells.
Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human colorectal cancer shows strong cytoplasmic immunoreactivity in tumor cells.
Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human fallopian tube shows strong cytoplasmic positivity in epithelial cells.
Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human placenta shows strong staining in the trophoblast.
Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human skeletal muscle shows absence of immunoreactivity (negative control).
Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody (CL1111) [NBP2-52966] - Staining of human testis shows strong cytoplasmic immunoreactivity in seminiferous tubules cells.

Applications for DIAPH-2 Antibody (CL1111) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DIAPH-2

DIAPH2 may play a role in the development and normal function of the ovaries. Mutations of this gene have been linked to premature ovarian failure. Alternative splicing results in two protein isoforms. Furthermore, each splice variant undergoes additional splicing in the 3' UTR. Overall 4 splice variants have been described.

Long Name

Diaphanous Homolog 2

Alternate Names

DIA2, DIAPH2, DRF2, POF2

Gene Symbol

DIAPH2

Additional DIAPH-2 Products

Product Documents for DIAPH-2 Antibody (CL1111) - BSA Free

Certificate of Analysis

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Product Specific Notices for DIAPH-2 Antibody (CL1111) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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