Dkk-3 Antibody

Novus Biologicals | Catalog # NB100-899

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Dkk-3 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-AAAALLGGEEI corresponding to C-Terminus according to NP_037385.2, NP_056965.3, NP_001018067.1.

Epitope

C-AAAALLGGEEI

Specificity

NP_037385.2; NP_056965.3 and NP_001018067.1 are varients of the same protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Dkk-3 Antibody

Western Blot: Dkk-3 Antibody [NB100-899]

Western Blot: Dkk-3 Antibody [NB100-899]

Western Blot: Dkk-3 Antibody [NB100-899] - Staining of Human Cerebellum lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 2 ug/mL. Detected by chemiluminescence.

Applications for Dkk-3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/mL
Application Notes
Western blot: Approx 38kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 38.4 kDa according to NP_037385.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dkk-3

DKK3 is a secreted protein that is a member of the dickkopf family. It contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene.

Long Name

Dickkopf-3

Alternate Names

Dkk3, REIC

Entrez Gene IDs

27122 (Human)

Gene Symbol

DKK3

UniProt

NP_037385.2 (Human)

Additional Dkk-3 Products

Product Documents for Dkk-3 Antibody

Certificate of Analysis

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Product Specific Notices for Dkk-3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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