Dkk-4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-47484

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Dkk-4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PILERQLDEQDGTHAEGTTGHPVQENQPKRKPSIKKSQGRKGQEGESCLRTFDCGPGLCCARH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Dkk-4 Antibody - BSA Free (NBP2-47484) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Dkk-4 Antibody - BSA Free

Western Blot: Dkk-4 Antibody [NBP2-47484]

Western Blot: Dkk-4 Antibody [NBP2-47484]

Western Blot: Dkk-4 Antibody [NBP2-47484] - Analysis in control (vector only transfected HEK293T lysate) and DKK4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry: Dkk-4 Antibody [NBP2-47484]

Immunohistochemistry: Dkk-4 Antibody [NBP2-47484]

Immunohistochemistry: Dkk-4 Antibody [NBP2-47484] - Staining of human cerebellum shows distinct cytoplasmic positivity in Purkinje cells.

Applications for Dkk-4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dkk-4

DKK4 is a secreted protein that is a member of the dickkopf family. Like some other members of the dickkopf family DKK4 contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The activity of DKK4 is modulated by binding to the Wnt co-receptor and the co-factor kremen 2, and it is proposed that it is also an inhibitor of the Wnt signaling pathway.

Long Name

Dickkopf-4

Alternate Names

Dkk4

Gene Symbol

DKK4

Additional Dkk-4 Products

Product Documents for Dkk-4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Dkk-4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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