DLC1 Antibody - Isoform 1 and 3

Novus Biologicals | Catalog # NB300-917

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DLC1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence SVAIRKRSWEEHC corresponding to N-Terminus according to NP_872584.2, NP_079043.3.

Specificity

This antibody is expected to recognise isoforms 1 and 3 ( NP_872584.2 and NP_079043.3 resp) but NOT isoform 2 (NP_006085.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DLC1 Antibody - Isoform 1 and 3

Immunohistochemistry-Paraffin: DLC1 Antibody - Isoform 1 and 3 [NB300-917]

Immunohistochemistry-Paraffin: DLC1 Antibody - Isoform 1 and 3 [NB300-917]

Immunohistochemistry-Paraffin: DLC1 Antibody - Isoform 1 and 3 [NB300-917] - Negative Control showing staining of paraffin embedded Mouse Adrenal Gland, with no primary antibody.
Immunohistochemistry-Paraffin: DLC1 Antibody - Isoform 1 and 3 [NB300-917]

Immunohistochemistry-Paraffin: DLC1 Antibody - Isoform 1 and 3 [NB300-917]

Immunohistochemistry-Paraffin: DLC1 Antibody - Isoform 1 and 3 [NB300-917] - Staining of paraffin embedded Mouse Adrenal Gland. Antibody at 4 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunofluorescence: DLC1 Antibody - Isoform 1 and 3 [NB300-917]

Immunofluorescence: DLC1 Antibody - Isoform 1 and 3 [NB300-917]

Immunofluorescence: DLC1 Antibody - Isoform 1 and 3 [NB300-917] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunofluorescence: DLC1 Antibody - Isoform 1 and 3 [NB300-917]

Immunofluorescence: DLC1 Antibody - Isoform 1 and 3 [NB300-917]

Immunofluorescence: DLC1 Antibody - Isoform 1 and 3 [NB300-917] - Analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for DLC1 Antibody - Isoform 1 and 3

Application
Recommended Usage

Immunohistochemistry

4 ug/mL

Immunohistochemistry-Paraffin

4 ug/mL
Application Notes
WB successful usage for human heart is reported in scientific literature (PMID: 19874489). IHC-P: Human adrenal gland (medulla) shows strong reticulate staining in the cytoplasm of medular cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DLC1

This gene is deleted in the primary tumor of hepatocellular carcinoma. It maps to 8p22-p21.3, a region frequently deleted in solid tumors. It is suggested that this gene is a candidate tumor suppressor gene for human liver cancer, as well as for prostate, lung, colorectal, and breast cancers. Alternative splicing at this locus results in several transcript variants encoding different isoforms.

Alternate Names

ARHGAP7 STARD12 deleted in liver cancer 1 variant 2, deleted in liver cancer 1, Deleted in liver cancer 1 protein, DLC-1, FLJ21120, HP, HP protein, KIAA1723, p122-RhoGAP, rho GTPase-activating protein 7, Rho-GTPase-activating protein 7, Rho-type GTPase-activating protein 7, StARD12, StAR-related lipid transfer (START) domain containing 12, StAR-related lipid transfer protein 12, START domain-containing protein 12

Entrez Gene IDs

10395 (Human); 50768 (Mouse)

Gene Symbol

DLC1

UniProt

NP_079043 (Human), NP_872584.2 (Human)

Additional DLC1 Products

Product Documents for DLC1 Antibody - Isoform 1 and 3

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Product Specific Notices for DLC1 Antibody - Isoform 1 and 3

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DLC1 Antibody - Isoform 1 and 3

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