DLX5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85793

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RRVPSIRSGDFQAPFQTSAAMHHPSQESPTLPESSATDSDYYSPTGGAPHGYCSPTSASYGKALNPYQYQYHGVNGSAGSYPAKAYADYSYASSYHQYGGAYNRVPSATNQPEKEVTEPEVRMVNGKPKKVRKP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DLX5 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DLX5 Antibody [NBP1-85793]

Immunocytochemistry/ Immunofluorescence: DLX5 Antibody [NBP1-85793]

Immunocytochemistry/Immunofluorescence: DLX5 Antibody [NBP1-85793] - Staining of human cell line U-2 OS shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-85793]

Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-85793]

Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-85793] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-85793]

Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-85793]

Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-85793] - Staining of human endometrium shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-85793]

Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-85793]

Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-85793] - Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-85793]

Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-85793]

Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-85793] - Staining of human placenta shows weak nuclear positivity in trophoblastic cells.
DLX5 Antibody - BSA Free

Western Blot: DLX5 Antibody - BSA Free [NBP1-85793] -

Expression and enhancer association define two classes of EVT regulators.a Number of genes (blue), TFs (green), and SE-associated TFs (red) in class 2 and 3 genes. b Gene track view of H3K27ac signals near the candidate regulators of EVT differentiation, with red bars indicating SEs. c Expression patterns of early- and late-stage TF candidates during EVT differentiation. Values represent log2-transformed counts compared to the average. d Enriched motifs within the top 30% of H3K27ac peaks in EVT on differentiation day 3 were identified using the findMotifsGenome.pl module under HOMER (v4.11). p-values were calculated based on the default settings of the module, with a region size of 500 bp. e Western blot analysis demonstrating the expression patterns of EVT regulator candidates in CT27 TSC line. HLA-G expression was measured to validate EVT differentiation. ACTB was used as a loading control. *ZNF439 (lower band). Independent repeats, utilizing other TSC lines, were conducted as presented in Supplementary Fig. 2d. f Normalized transcripts per million value (nTPM) of EVT regulator candidates in scRNA-seq data of human first-trimester placenta (retrieved from the Human Protein Atlas19,102). Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41467-024-45669-2), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
DLX5 Antibody - BSA Free Chromatin Immunoprecipitation ChIP: DLX5 Antibody - BSA Free

Chromatin Immunoprecipitation ChIP: DLX5 Antibody - BSA Free

ChIP-Exo-Seq composite graph for Anti-DLX5 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.

Applications for DLX5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DLX5

Dlx5 (distal-less homeobox 5) gene is a member of a homeobox gene family similiar to the Drosophila distal-less gene. The encoded Dlx5 protein is localized to the nucleus where it functions as a transcriptional regulator during neural development. In the developing CNS, Dlx5 is one of the earliest known markers before the formation of an overt neural plate. During late gastrulation Dlx5 (gene) expression becomes localized to the anterior neural ridge, which defines the rostral boundary of the neural plate, and also extends caudolaterally, marking the region of the presumptive neural crest. Subsequently, Dlx5 is expressed in tissues (olfactory epithelium, ventral cephalic epithelium) that are believed to derive from the anterior neural ridge.

Long Name

Distal-less Homeobox 5

Alternate Names

distal-less homeo box 5, distal-less homeobox 5, homeobox protein DLX-5

Gene Symbol

DLX5

Additional DLX5 Products

Product Documents for DLX5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DLX5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DLX5 Antibody - BSA Free

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Protocols

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