DNA Ligase III Antibody

Name: DNA Ligase III Antibody
Brand: Novus Biologicals
SKU: NB100-81661
Price: 459 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-81661

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 800 and 850 of human DNA Ligase III using the numbering given in entry CAA59230.1 (GeneID 3980).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNA Ligase III Antibody

Western Blot: DNA Ligase III Antibody [NB100-81661]

Western Blot: DNA Ligase III Antibody [NB100-81661] - Detection of Human DNL3 by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNL3 antibody NB100-81661 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: DNA Ligase III Antibody [NB100-81661]

Immunoprecipitation: DNA Ligase III Antibody [NB100-81661] - Detection of human DNA Ligase III by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNA Ligase III antibody NB100-81661 used for IP at 6 ug per reaction. DNA Ligase III was also immunoprecipitated by rabbit anti-DNA Ligase III antibody from Company B. For blotting immunoprecipitated DNA Ligase III, NB100-81661 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for DNA Ligase III Antibody

Application

Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DNA Ligase III

This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

DNA ligase 3

Alternate Names

LIG3

Entrez Gene IDs

3980 (Human)

Gene Symbol

LIG3

UniProt

P49916 (Human)

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Product Documents for DNA Ligase III Antibody

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Product Specific Notices for DNA Ligase III Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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