DNA-PKcs [p Ser2056] Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-05444

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DNA-PKcs Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S2056 of human DNA-PKcs (NP_008835.5). SYSSQDPRPA

Modification

p Ser2056

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNA-PKcs [p Ser2056] Antibody - BSA Free

Western Blot: DNA-PKcs [p Ser2056] AntibodyAzide and BSA Free [NBP3-05444]

Western Blot: DNA-PKcs [p Ser2056] AntibodyAzide and BSA Free [NBP3-05444]

Western Blot: DNA-PKcs [p Ser2056] Antibody [NBP3-05444] - Western blot analysis of extracts of HeLa cells, using DNA-PKcs antibody (NBP3-05444). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Immunocytochemistry/ Immunofluorescence: DNA-PKcs [p Ser2056] Antibody - Azide and BSA Free [NBP3-05444]

Immunocytochemistry/ Immunofluorescence: DNA-PKcs [p Ser2056] Antibody - Azide and BSA Free [NBP3-05444]

Immunocytochemistry/Immunofluorescence: DNA-PKcs [p Ser2056] Antibody [NBP3-05444] - Immunofluorescence analysis of U2OS cells using DNA-PKcs antibody (NBP3-05444) at dilution of 1:100. Blue: DAPI for nuclear staining.
DNA-PKcs [p Ser2056] Antibody - Azide and BSA Free

Immunohistochemistry: DNA-PKcs [p Ser2056] Antibody - Azide and BSA Free [DNA-PKcs] -

Immunohistochemistry: DNA-PKcs [p Ser2056] Antibody - Azide and BSA Free [DNA-PKcs] - Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using DNA-PKcs Rabbit pAb at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for DNA-PKcs [p Ser2056] Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DNA-PKcs

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.(supplied by OMIM)

Long Name

DNA-dependent Protein Kinase Catalytic Subunit

Alternate Names

DNAPK, DNAPKcs, DNPK1, HYRC1, p350, PRKDC, XRCC7

Gene Symbol

PRKDC

Additional DNA-PKcs Products

Product Documents for DNA-PKcs [p Ser2056] Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DNA-PKcs [p Ser2056] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

DNA Repair

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Protocols

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