DNA polymerase theta Antibody (1C11) - Azide and BSA Free

Novus Biologicals | Catalog # H00010721-M09

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1C11

Format

Azide and BSA Free
View all DNA polymerase theta Primary Antibodies »

Product Specifications

Immunogen

POLQ (NP_006587, 2572 a.a. ~ 2680 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDRTGLSRKRKLQGMFCPIRGGFFILQLHDELLYE

Specificity

POLQ - polymerase (DNA directed), theta (1C11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for DNA polymerase theta Antibody (1C11) - Azide and BSA Free

Western Blot: DNA polymerase theta Antibody (1C11) [H00010721-M09]
ELISA: DNA polymerase theta Antibody (1C11) [H00010721-M09]

ELISA: DNA polymerase theta Antibody (1C11) [H00010721-M09]

ELISA: DNA polymerase theta Antibody (1C11) [H00010721-M09] - Detection limit for recombinant GST tagged POLQ is approximately 1ng/ml as a capture antibody.

Applications for DNA polymerase theta Antibody (1C11) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reviewed Applications

Read 1 review rated 5 using H00010721-M09 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DNA polymerase theta

DNA polymerase theta, also referred to as POLQ, is highly expressed in the testes. POLQ has a 2,590 amino acid long isoform that is approximately 290kDa and a shorter 1,786 amino acid long isoform that is approximately 198kDa. POLQ is highly expressed in the testes, plays a role in DNA polymerase activity, and may be implicated in interstrand crosslink repair (ICR). Research on DNA polymerase theta has shown a connection to several diseases and disorders including stomach cancer, malaria, breast cancer, tuberculosis, and meningioma. POLQ has also been shown to have interactions with AP2S1.

Alternate Names

DKFZp781A0112, DNA polymerase eta, EC 2.7.7.7, POLHDNA polymerase theta, polymerase (DNA directed), theta, PRO0327

Entrez Gene IDs

10721 (Human)

Gene Symbol

POLQ

Additional DNA polymerase theta Products

Product Documents for DNA polymerase theta Antibody (1C11) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DNA polymerase theta Antibody (1C11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DNA polymerase theta Antibody (1C11) - Azide and BSA Free

Customer Reviews for DNA polymerase theta Antibody (1C11) - Azide and BSA Free (1)

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  • DNA polymerase theta Antibody (1C11)
    Name: Kara Chan
    Application: Western Blot
    Sample Tested: Purified protein, Hela whole cell lysate, cell line, whole cell lysate and Whole HeLa and nuclear extract
    Species: Human
    Verified Customer | Posted 08/29/2017
    western blot for purified C-terminal pol theta
    1:1000
    DNA polymerase theta Antibody (1C11) - Azide and BSA Free H00010721-M09

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