DNMT1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-84328
![DNMT1 Antibody - BSA Free Immunohistochemistry-Paraffin: DNMT1 Antibody - BSA Free [NBP1-84328]](https://resources.rndsystems.com/images/products/nbp1-84328_rabbit-polyclonal-dnmt1-antibody-10420251538680.jpg "Immunohistochemistry-Paraffin: DNMT1 Antibody - BSA Free [NBP1-84328]")
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: IPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVL
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DNMT1 Antibody - BSA Free
Immunohistochemistry-Paraffin: DNMT1 Antibody - BSA Free [NBP1-84328]
Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.![DNMT1 Antibody - BSA Free Immunohistochemistry-Paraffin: DNMT1 Antibody - BSA Free [NBP1-84328]](https://resources.rndsystems.com/images/products/nbp1-84328_rabbit-polyclonal-dnmt1-antibody-10420251538649.jpg "Immunohistochemistry-Paraffin: DNMT1 Antibody - BSA Free [NBP1-84328]")
Immunohistochemistry-Paraffin: DNMT1 Antibody - BSA Free [NBP1-84328]
Staining of human pancreas shows no positivity in exocrine glandular cells as expected.![DNMT1 Antibody - BSA Free Immunohistochemistry-Paraffin: DNMT1 Antibody - BSA Free [NBP1-84328]](https://resources.rndsystems.com/images/products/nbp1-84328_rabbit-polyclonal-dnmt1-antibody-104202515321342.jpg "Immunohistochemistry-Paraffin: DNMT1 Antibody - BSA Free [NBP1-84328]")
Immunohistochemistry-Paraffin: DNMT1 Antibody - BSA Free [NBP1-84328]
Staining of human lymph node shows strong nuclear positivity in germinal center cells.![DNMT1 Antibody - BSA Free Immunohistochemistry-Paraffin: DNMT1 Antibody - BSA Free [NBP1-84328]](https://resources.rndsystems.com/images/products/nbp1-84328_rabbit-polyclonal-dnmt1-antibody-104202515403130.jpg "Immunohistochemistry-Paraffin: DNMT1 Antibody - BSA Free [NBP1-84328]")
![DNMT1 Antibody - BSA Free Immunohistochemistry-Paraffin: DNMT1 Antibody - BSA Free [NBP1-84328]](https://resources.rndsystems.com/images/products/nbp1-84328_rabbit-polyclonal-dnmt1-antibody-10420251540313.jpg "Immunohistochemistry-Paraffin: DNMT1 Antibody - BSA Free [NBP1-84328]")
Immunohistochemistry-Paraffin: DNMT1 Antibody - BSA Free [NBP1-84328]
Staining of human placenta shows strong nuclear positivity in cytotrophoblast.Applications for DNMT1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DNMT1
Improper methylation patterns have been linked to certain human tumors and developmental abnormalities. DNMT1 antibodies are useful tools for DNA methlyation research and studies on certian cancer.
Long Name
DNA [Cytosine-5-]-Methyltransferase 1
Alternate Names
CXXC9, MCMT
Gene Symbol
DNMT1
Additional DNMT1 Products
Product Documents for DNMT1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for DNMT1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for DNMT1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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