Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 64B814.1

Format

BSA Free
View all DNMT3A Primary Antibodies »

Product Specifications

Immunogen

This DNMT3A Antibody (64B814.1) was raised against bacteria expressed HIS-tag recombinant mouse Dnmt3a.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 16631596).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for DNMT3A Antibody (64B814.1) - BSA Free

Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521]

Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521]

Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis of Dnmt3a in (A) Dnmt3a transfected and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml.
Flow (Intracellular): DNMT3A Antibody (64B814.1) [NB100-56521]

Flow (Intracellular): DNMT3A Antibody (64B814.1) [NB100-56521]

Flow (Intracellular): DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis of Alexa Fluor (R) 488 conjugate of NB100-56521. An intracellular stain was performed on HeLa cells with DNMT3A antibody (64B814.1) NB100-56521AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange).

Applications for DNMT3A Antibody (64B814.1) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Western Blot

1-3 ug/ml

Reviewed Applications

Read 1 review rated 5 using NB100-56521 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNMT3A

Methylation of cytosine residues is essential for mammalian development by regulating gene expression, gene silencing and genomic imprinting (1). This process is controlled by the enzyme DNA (cytosine-5)-methyltransferase 3A (DNMt3a), which is encoded by the DNMT3A gene and has a predicted molecular weight of 102 kDa. DNA methyltransferases including DNMt3a are involved in de novo cytosine methylation and maintenance of embryonic and somatic cells through the transfer of methyl groups to specific CpG structures in DNA (2). Somatic mutations in DNMT3A are responsible for cytogenetically normal acute myeloid leukemia (CN-AML). Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA are linked with cancer (3,4). Mutations in the DNMT3A gene have also been found to cause DNMT3A overgrowth syndrome and myelodysplastic syndromes.

References

1. Thakur, A., Mackin, S. J., Irwin, R. E., O'Neill, K. M., Pollin, G., & Walsh, C. (2016). Widespread recovery of methylation at gametic imprints in hypomethylated mouse stem cells following rescue with DNMT3A2. Epigenetics Chromatin, 9, 53. doi:10.1186/s13072-016-0104-2

2. Ravichandran, M., Lei, R., Tang, Q., Zhao, Y., Lee, J., Ma, L.,... Dawlaty, M. M. (2019). Rinf Regulates Pluripotency Network Genes and Tet Enzymes in Embryonic Stem Cells. Cell Rep, 28(8), 1993-2003.e1995. doi:10.1016/j.celrep.2019.07.080

3. Pang, Y., Liu, J., Li, X., Xiao, G., Wang, H., Yang, G.,... Ren, H. (2018). MYC and DNMT3A-mediated DNA methylation represses microRNA-200b in triple negative breast cancer. J Cell Mol Med, 22(12), 6262-6274. doi:10.1111/jcmm.13916

4. Xia, L., Huang, W., Bellani, M., Seidman, M. M., Wu, K., Fan, D.,... Baylin, S. B. (2017). CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell, 31(5), 653-668.e657. doi:10.1016/j.ccell.2017.04.005

Long Name

DNA [Cytosine-5-]-Methyltransferase 3A

Alternate Names

DNA (cytosine-5-)-methyltransferase 3 alpha, DNA (cytosine-5)-methyltransferase 3A, DNA cytosine methyltransferase 3A2, DNA Methyltransferase 3 Alpha, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, Dnmt3a, DNMT3A2, EC 2.1.1.37, M.HsaIIIA, TBRS

Gene Symbol

DNMT3A

Additional DNMT3A Products

Product Documents for DNMT3A Antibody (64B814.1) - BSA Free

Certificate of Analysis

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Product Specific Notices for DNMT3A Antibody (64B814.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adult Neurogenesis
Epigenetics

Citations for DNMT3A Antibody (64B814.1) - BSA Free

Customer Reviews for DNMT3A Antibody (64B814.1) - BSA Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Lysate from homogenized mouse brain cells
    Species: Mouse
    Verified Customer | Posted 07/11/2014
    WB for Dnmt3a in mouse brain cells
    DNMT3A Antibody (64B814.1) - BSA Free NB100-56521

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Protocols

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FAQs for DNMT3A Antibody (64B814.1) - BSA Free

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  • Q: I ordered the Dnmt3a Antibody (64B814.1) and would like to use it in IF and eventually intracellular FACS staining. I wanted to know which conditions did you uses in ICC/IF since it is written in the data sheet that it has been tested for this application.

    A: Our ICC protocol can be found at this link.

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