Dnmt3L Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00029947-D01P

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Dnmt3L Primary Antibodies »

Product Specifications

Immunogen

DNMT3L (AAH02560.1, 1 a.a. - 386 a.a.) full-length human protein. MAAIPALDPEAEPSMDVILVGSSELSSSVSPGTGRDLIAYEVKANQRNIEDICICCGSLQVHTQHPLFEGGICAPCKDKFLDALFLYDDDGYQSYCSICCSGETLLICGNPDCTRCYCFECVDSLVGPGTSGKVHAMSNWVCYLCLPSSRSGLLQRRRKWRSQLKAFYDRESENPLEMFETVPVWRRQPVRVLSLFEDIKKELTSLGFLESGSDPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPPLGHTCDRPPSWYLFQFHRLLQYARPKPGSPGPFFWMFVDNLVLNKEDLDVASRFLEMEPVTIPDVHGGSLQNAVRVWSNIPAIRSRHWALVSEEELSLLAQNKQSSKLAAKWPTKLVKNCFLPLREYFKYFSTELTSSL

Specificity

Reacts with DNA (cytosine-5-)-methyltransferase 3-like.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dnmt3L Antibody - Azide and BSA Free

Western Blot: Dnmt3L Antibody [H00029947-D01P]

Western Blot: Dnmt3L Antibody [H00029947-D01P]

Western Blot: Dnmt3L Antibody [H00029947-D01P] - Analysis of DNMT3L expression in mouse liver.
Immunocytochemistry/ Immunofluorescence: Dnmt3L Antibody [H00029947-D01P]

Immunocytochemistry/ Immunofluorescence: Dnmt3L Antibody [H00029947-D01P]

Immunocytochemistry/Immunofluorescence: Dnmt3L Antibody [H00029947-D01P] - Analysis of antibody to DNMT3L on HeLa cell. Antibody concentration 30 ug/ml.
Western Blot: Dnmt3L Antibody [H00029947-D01P]

Western Blot: Dnmt3L Antibody [H00029947-D01P]

Western Blot: Dnmt3L Antibody [H00029947-D01P] - Analysis of DNMT3L expression in human liver.
Western Blot: Dnmt3L Antibody [H00029947-D01P]

Western Blot: Dnmt3L Antibody [H00029947-D01P]

Western Blot: Dnmt3L Antibody [H00029947-D01P] - Analysis of DNMT3L expression in transfected 293T cell line by DNMT3L polyclonal antibody.Lane 1: DNMT3L transfected lysate(43.50 KDa).Lane 2: Non-transfected lysate.

Applications for Dnmt3L Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against tissue and transfected lysate in western blot, and as a detection antibody in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Dnmt3L

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined. [provided by RefSeq]

Alternate Names

DNA (cytosine-5-)-methyltransferase 3-like, DNA (cytosine-5)-methyltransferase 3-like, human cytosine-5-methyltransferase 3-like protein, 10cytosine-5-methyltransferase 3-like protein, MGC1090

Entrez Gene IDs

29947 (Human)

Gene Symbol

DNMT3L

UniProt

AAH02560.1 (Human)

Additional Dnmt3L Products

Product Documents for Dnmt3L Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Dnmt3L Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Dnmt3L Antibody - Azide and BSA Free

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Protocols

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FAQs

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