Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25444

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1584 expressed in HEK293

Format

Azide and BSA Free
View all Dopamine D2 R/DRD2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Dopamine D2R/DRD2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%), Porcine (88%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free

Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free [NBP3-25444] -

Immunocytochemistry/Immunofluorescence: Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free [NBP3-25444] - Dopamine Receptor D2 antibody [HL1584] detects Dopamine Receptor D2 protein by immunofluorescent analysis. Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1584] (NBP3-25444) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI.
Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free

Western Blot: Dopamine D2R/DRD2 Antibody (HL1584) [NBP3-25444] -

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1584] (NBP3-25444) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free

Dopamine D2R/DRD2 Antibody (HL1584) -

Dopamine Receptor D2 antibody [HL1584] detects DRD2 protein by flow cytometry analysis.Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with 0.1% saponin buffer to permeabilize the cell membrane.The cells were following stained with Dopamine Receptor D2 antibody [HL1584] diluted at 1:50 or a Rabbit IgG isotype control control at 4ºC for 1 hour.

Applications for Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Assay dependent

Immunocytochemistry/ Immunofluorescence

Assay dependent

Western Blot

1:500-1:3000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dopamine D2 R/DRD2

The DRD2 Dopamine Receptor is involved in the neuromodulation of appetitive behaviors. DRD2 may be linked to alcoholism and other substance-use disorders. Mutations in DRD2 in mice lead to the suppression of reward behavior with morphine and to the reduction of spontaneous movements, a phenotype resembling Parkinson's disease. Three alternatively spliced isoforms have been identified. DRD2 has been reported to be expressed in various regions of the brain, as well as in aorta, artery, eye, and spinal cord. ESTs have been isolated from normal pituitary and in brain, colon, lung, pancreas, and skeletal muscle cancer libraries.

Long Name

Dopamine D2 Receptor

Alternate Names

D2DR, D2R, Dopamine D2R, DRD2

Gene Symbol

DRD2

Additional Dopamine D2 R/DRD2 Products

Product Documents for Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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