Dopamine D2R/DRD2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-88996

Novus Biologicals
Discontinued Product
NBP2-88996 has been discontinued. View all Dopamine D2 R/DRD2 products.

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Avian

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free
View all Dopamine D2 R/DRD2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the sequence near the N-terminus of mouse Dopamine D2R/DRD2.

Reactivity Notes

Predicted species reactivity based on epitope sequence homology: rhesus macaque, chimpanzee, horse, arctic fox, dog, opossum, ferret. Avian reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dopamine D2R/DRD2 Antibody - BSA Free

Western Blot: Dopamine D2R/DRD2 Antibody [NBP2-88996]

Western Blot: Dopamine D2R/DRD2 Antibody [NBP2-88996]

Western Blot: Dopamine D2R/DRD2 Antibody [NBP2-88996] - MW marker (1) and 10ug of rat heart membrane extract (2) probed with Dopamine Receptor (D2) pAb at 4.0ug/ml.
Immunohistochemistry: Dopamine D2R/DRD2 Antibody [NBP2-88996]

Immunohistochemistry: Dopamine D2R/DRD2 Antibody [NBP2-88996]

Immunohistochemistry: Dopamine D2R/DRD2 Antibody [NBP2-88996] - Rat cerebral cortex. Neurons are labeled with Dopamine Receptor (D2) pAb in conjunction with Cy3 conjugated rabbit IgG antibody (H+L).
ELISA: Dopamine D2R/DRD2 Antibody [NBP2-88996]

ELISA: Dopamine D2R/DRD2 Antibody [NBP2-88996]

ELISA: Dopamine D2R/DRD2 Antibody [NBP2-88996] - HEK293 cells expressing dopamine receptor (D2) were treated with indicated concentrations of agonist (Quinpirol) and antagonist (Siperone) and probed with Dopamine Receptor (D2) pAb (1:1000 of a stock solution of 1ug/uL) by cellular ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean -+SEM (n=3).
Dopamine D2R/DRD2 Antibody

Western Blot: Dopamine D2R/DRD2 Antibody [NBP2-88996] -

Western Blot: Dopamine D2R/DRD2 Antibody [NBP2-88996] - Dopamine D2R/DRD2 antibody [NBP2-88996]. Left image is the MW marker and right is two replicates of ~20 ug protein extract from one hemisphere of house sparrow brain tissue stained with D2 primary antibody at 1.0 ug/ml. Image from verified customer review.

Applications for Dopamine D2R/DRD2 Antibody - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-88996 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS containing 50% glycerol

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dopamine D2 R/DRD2

The DRD2 Dopamine Receptor is involved in the neuromodulation of appetitive behaviors. DRD2 may be linked to alcoholism and other substance-use disorders. Mutations in DRD2 in mice lead to the suppression of reward behavior with morphine and to the reduction of spontaneous movements, a phenotype resembling Parkinson's disease. Three alternatively spliced isoforms have been identified. DRD2 has been reported to be expressed in various regions of the brain, as well as in aorta, artery, eye, and spinal cord. ESTs have been isolated from normal pituitary and in brain, colon, lung, pancreas, and skeletal muscle cancer libraries.

Long Name

Dopamine D2 Receptor

Alternate Names

D2DR, D2R, Dopamine D2R, DRD2

Gene Symbol

DRD2

Additional Dopamine D2 R/DRD2 Products

Product Documents for Dopamine D2R/DRD2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Dopamine D2R/DRD2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Dopamine D2R/DRD2 Antibody - BSA Free (1)

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Customer Images

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  • Dopamine D2R/DRD2 Antibody
    Name: Melanie Kimball
    Application: Western Blot
    Sample Tested: Adult brain
    Species: Avian and House sparrow
    Verified Customer | Posted 06/16/2023
    Western Blot: Dopamine D2R/DRD2 antibody [NBP2-88996]. Left image is the MW marker and right is two replicates of ~20 ug protein extract from one hemisphere of house sparrow brain tissue stained with D2 primary antibody at 1.0 ug/ml.
    Dopamine D2R/DRD2 Antibody - BSA Free NBP2-88996
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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FAQs

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