Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2D9

Format

Azide and BSA Free
View all DPP3 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human DPP3(NP_005691) produced in HEK293 cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

82.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse DPP3 Antibody (OTI2D9) - Azide and BSA Free (NBP2-01007) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for DPP3 Antibody (OTI2D9) - Azide and BSA Free

Western Blot: DPP3 Antibody (OTI2D9)Azide and BSA Free [NBP2-71541]

Western Blot: DPP3 Antibody (OTI2D9)Azide and BSA Free [NBP2-71541]

Western Blot: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP3.
Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541]

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541]

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - Staining of paraffin-embedded Human lymphoma tissue using anti-DPP3 mouse monoclonal antibody.
Flow Cytometry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541]

Flow Cytometry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541]

Flow Cytometry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-DPP3 antibody, and then analyzed by flow cytometry.
Western Blot: DPP3 Antibody (OTI2D9)Azide and BSA Free [NBP2-71541]

Western Blot: DPP3 Antibody (OTI2D9)Azide and BSA Free [NBP2-71541]

Western Blot: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - Analysis of extracts (35ug) from 9 different cell lines by using anti-DPP3 monoclonal antibody.
Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541]

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541]

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DPP3 mouse monoclonal antibody.
Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541]

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541]

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DPP3 mouse monoclonal antibody.
Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541]

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541]

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DPP3 mouse monoclonal antibody.
Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541]

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541]

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - Staining of paraffin-embedded Human lymph node tissue using anti-DPP3 mouse monoclonal antibody.
Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541]

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541]

Immunohistochemistry: DPP3 Antibody (OTI2D9) - Azide and BSA Free [NBP2-71541] - Staining of paraffin-embedded Human prostate tissue using anti-DPP3 mouse monoclonal antibody.

Applications for DPP3 Antibody (OTI2D9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DPP3

DPP3 encodes a protein that is a member of the S9B family in clan SC of the serine proteases. This cytoplasmic protein binds a single zinc ion with its zinc-binding motif (HELLGH) and has post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Increased activity of this protein is associated with endometrial and ovarian cancers. Alternate transcriptional splice variants have been characterized. [provided by RefSeq]

Long Name

Dipeptidyl-peptidase 3

Alternate Names

DPPIII

Gene Symbol

DPP3

Additional DPP3 Products

Product Documents for DPP3 Antibody (OTI2D9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DPP3 Antibody (OTI2D9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Metalloproteases and Regulators

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Protocols

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FAQs

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