DPP9 Antibody (OTI2E3)

Novus Biologicals | Catalog # NBP2-01521

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2E3
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Product Specifications

Immunogen

Full length human recombinant protein of human DPP9(NP_631898) produecd in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

96.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DPP9 Antibody (OTI2E3)

Western Blot: DPP9 Antibody (2E3) [NBP2-01521] Analysis of extracts (35ug) from 9 different cell lines by using anti-DPP9 monoclonal antibody.
Immunohistochemistry-Paraffin: DPP9 Antibody (OTI2E3) [NBP2-01521]

Immunohistochemistry-Paraffin: DPP9 Antibody (OTI2E3) [NBP2-01521]

Immunohistochemistry-Paraffin: DPP9 Antibody (2E3) [NBP2-01521] - Staining of paraffin-embedded Human lymphoma tissue using anti-DPP9 mouse monoclonal antibody.
Flow Cytometry: DPP9 Antibody (OTI2E3) [NBP2-01521]

Flow Cytometry: DPP9 Antibody (OTI2E3) [NBP2-01521]

Flow Cytometry: DPP9 Antibody (2E3) [NBP2-01521] - Analysis of Jurkat cells, using anti-DPP9 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: DPP9 Antibody (OTI2E3) [NBP2-01521]

Western Blot: DPP9 Antibody (OTI2E3) [NBP2-01521]

Western Blot: DPP9 Antibody (2E3) [NBP2-01521] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP9.
Immunohistochemistry-Paraffin: DPP9 Antibody (OTI2E3) [NBP2-01521]

Immunohistochemistry-Paraffin: DPP9 Antibody (OTI2E3) [NBP2-01521]

Immunohistochemistry-Paraffin: DPP9 Antibody (2E3) [NBP2-01521] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DPP9 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DPP9 Antibody (OTI2E3) [NBP2-01521]

Immunohistochemistry-Paraffin: DPP9 Antibody (OTI2E3) [NBP2-01521]

Immunohistochemistry-Paraffin: DPP9 Antibody (2E3) [NBP2-01521] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DPP9 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DPP9 Antibody (OTI2E3) [NBP2-01521]

Immunohistochemistry-Paraffin: DPP9 Antibody (OTI2E3) [NBP2-01521]

Immunohistochemistry-Paraffin: DPP9 Antibody (2E3) [NBP2-01521] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DPP9 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DPP9 Antibody (OTI2E3) [NBP2-01521]

Immunohistochemistry-Paraffin: DPP9 Antibody (OTI2E3) [NBP2-01521]

Immunohistochemistry-Paraffin: DPP9 Antibody (2E3) [NBP2-01521] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DPP9 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DPP9 Antibody (OTI2E3) [NBP2-01521]

Immunohistochemistry-Paraffin: DPP9 Antibody (OTI2E3) [NBP2-01521]

Immunohistochemistry-Paraffin: DPP9 Antibody (2E3) [NBP2-01521] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DPP9 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: DPP9 Antibody (OTI2E3) [NBP2-01521]

Immunohistochemistry-Paraffin: DPP9 Antibody (OTI2E3) [NBP2-01521]

Immunohistochemistry-Paraffin: DPP9 Antibody (2E3) [NBP2-01521] - Staining of paraffin-embedded Human endometrium tissue using anti-DPP9 mouse monoclonal antibody.
DPP9 Antibody (OTI2E3)

DPP9 Antibody (OTI2E3)[NBP2-01521]

DPP9 Antibody (OTI2E3)[NBP2-01521]Simple Western� analysis of endogenous protein PFKP from MCF-7 lysates (0.1 mg/mL) using PFKP Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.
DPP9 Antibody (OTI2E3)

Western Blot: DPP9 Antibody (OTI2E3) [NBP2-01521] -

Dependence on HCK for DPP8/9 inhibitor-induced pyroptosis. (A) Expression level of DPP8, DPP9, caspase-1, CARD8, GSDMD, or caspase-3 in THP-1, MM.1S, KARPAS299, KG1, NAMALWA, or Daudi cells was estimated by Western blot analysis. (B) Gene expression of THP-1, MM.1S, KARPAS299, KG1, NAMALWA, or Daudi cells was analyzed by microarray method using 3D-Gene. (C) Expression level of HCK, LCK, Fyn, c-Fgr, Lyn, or Blk in THP-1, MM.1S, KARPAS299, KG1, NAMALWA, or Daudi cells was estimated by Western blot analysis. (D) 1.0 × 105 of hematological cancer cell lines (Jurkat, K562, MOLM-13, NOMO-1, RPMI8226, Raji, or SKM-1) were cultured with 1G244 at doses of 0–10 uM for 72 h. Cell number was estimated by a colorimetric assay using WST-1 reagent (n = 6). (E) Expression level of HCK or CARD8 in Jurkat, K562, MOLM-13, NOMO-1, RPMI8226, Raji, or SKM-1 cells was estimated by Western blot analysis. (F) Knockdown studies of HCK in MM.1S cells. NT, no treatment; GFP, control vector; KD, knockdown. Expression level of HCK was estimated by Western blot analysis. (G) 1.0 × 105 of MM.1S cells and their transfectants were cultured with DPP8/9 inhibitors (1G244 or talabostat) at doses of 0–100 uM for 6 h. Cytotoxicity was estimated by a LDH release assay (n = 6). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37048172), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for DPP9 Antibody (OTI2E3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.71 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DPP9

This gene encodes a protein that is a member of the S9B family in clan SC of the serine proteases. The protein has been shown to have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Although the activity of this protein is similar to that of dipeptidyl peptidase 4 (DPP4), it does not appear to be membrane bound. In general, dipeptidyl peptidases appear to be involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. Several transcript variants of this gene have been described but not fully characterized. [provided by RefSeq].

Long Name

Dipeptidyl peptidase 9

Alternate Names

DPLP9, DPRP2

Entrez Gene IDs

91039 (Human)

Gene Symbol

DPP9

Additional DPP9 Products

Product Documents for DPP9 Antibody (OTI2E3)

Certificate of Analysis

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Product Specific Notices for DPP9 Antibody (OTI2E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Citations for DPP9 Antibody (OTI2E3)

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Protocols

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