DPY30 Antibody - BSA Free

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• DPY30 Antibody

  Name: zhang yuan

  Application: Western Blot

  Sample Tested: SPCA-1 cells, H1299 whole cell lysate, SPCA-1 whole cell lysate and A549 whole cell lysate

  Species: Human

  Verified Customer | Posted 12/05/2018

  The figure above can be used as a reference for Western blot.

  These are the main steps of our western blot. The protein concentration was assayed using the BCA protein assay(Thermo REF:23227). For this,40 μg of protein per lanewas separated by 12% SDS-PAGE and electroblotted onto a PVDF membrane. Then, non-specific binding was blocked by incubating with5% non-fat milk in TBST buffer at room temperature for 2 h. PVDF membranes were incubated overnight at 4 °C with primary antibodies which were diluted in TBS-T with 5% non-fat milk as follows: DPY30 (1:200), GAPDH (1:3000). This was followed by incubation with the corresponding secondary antibody(1:5000) at room temperature for 1 h. Finally, antigens were detected using the standard chemical luminescence method (ECL).

  

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