DRAQ5 (TM)

Novus Biologicals | Catalog # NBP2-81125

Novus Biologicals
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Key Product Details

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Microscopy

Product Summary for DRAQ5 (TM)

Key Features of DRAQ5 (TM) :
  • Bright, far-red DNA dye that works on live or fixed cell and tissue preparations for multiple applications such as flow cytometry, fluorescence imaging, and high content screening
  • Proven no-lyse, no-wash method for measuring DNA content in cells
  • Tested on hundreds of cell and tissue types
  • Exceptionally photostable so no photobleaching effect
  • Convenient, ready-to-use formulation
  • Enables phenotyping of blood/BM and direct cellular DNA content analysis in flow cytometry without any RBC lysis or fixation, permeabilization or RNase treatment
  • Spectrally compatible with most visible-range fluorophores including GFP/FITC and R-PE

    • Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8C. DO NOT FREEZE. DRAQ5 (TM) can come out of solution when frozen and it is difficult (but not impossible) to get it back into solution.

    Product Specifications

    Applications

    Flow Cytometry (1:250 - 1:1000)
    Immunocytochemistry/ Immunofluorescence (1:1000)
    Live Imaging Microscopy (1:1000)
    Fluorescence Imaging (1:1000)

    Application Notes

    Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:34354753).

    Spectra Viewer

    Plan Your Experiments

    Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

    Spectra Viewer
    Spectra Viewer

    Scientific Data Images for DRAQ5 (TM)

    Live Imaging Microscopy: DRAQ5 (TM) [NBP2-81125]

    Live Imaging Microscopy: DRAQ5 (TM) [NBP2-81125]

    Live Imaging Microscopy: DRAQ5 (TM) [NBP2-81125] - DRAQ5 was diluted in EMEM culture media and applied to live HeLa cells at 5 uM (1:1000) for 30 minutes at room temperature and protected from light. Imaging was done immediately after staining without washing the cells.
    Immunocytochemistry/ Immunofluorescence: DRAQ5 (TM) [NBP2-81125]

    Immunocytochemistry/ Immunofluorescence: DRAQ5 (TM) [NBP2-81125]

    Immunocytochemistry/Immunofluorescence: DRAQ5 (TM) [NBP2-81125] - ' (red) counterstaining of fixed U2OS cells. AlexaFluor 488 antibody to beta-tubulin (green).
    Flow Cytometry: DRAQ5 (TM) [NBP2-81125]

    Flow Cytometry: DRAQ5 (TM) [NBP2-81125]

    Flow Cytometry: DRAQ5 (TM) [NBP2-81125] - DNA content analysis in live or fixed cells using DRAQ5 (TM)
    Immunocytochemistry/ Immunofluorescence: DRAQ5 (TM) [NBP2-81125]

    Immunocytochemistry/ Immunofluorescence: DRAQ5 (TM) [NBP2-81125]

    Immunocytochemistry/Immunofluorescence: DRAQ5 (TM) [NBP2-81125] - used to stain dsDNA of dividing cells.
    Flow Cytometry: DRAQ5 (TM) [NBP2-81125]

    Flow Cytometry: DRAQ5 (TM) [NBP2-81125]

    Flow Cytometry: DRAQ5 (TM) [NBP2-81125] - No lyse, no wash gating of nucleated cells from whole bone marrow gating on DRAQ5 (TM) signal.
    DRAQ5 (TM) [NBP2-81125] - Excitation and Emission Spectra for DRAQ5 (TM).

    Formulation, Preparation, and Storage

    Purification

    >97%

    Concentration

    Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

    Shipping

    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

    Storage

    Store at 4C in the dark. Do not freeze.

    Background: DRAQ5 (TM)

    Deep Red Anthraquinone 5 (DRAQ5 (TM)) is a cell-permeable dye belonging to the anthraquinone family that intercalates with high affinity to double stranded DNA (dsDNA). This far-red emitting fluorescent DNA dye is used for cell cycle analysis and nuclear staining. No measurable binding to mitochondrial DNA (mtDNA) has been detected and DRAQ5 (TM) shows negligible binding to RNA.

    The excitation wavelength maxima of DRAQ5 (TM) are 600 nm / 646 nm but it can also be suboptimally excited by the 488nm (blue laser) for flow cytometry. Its emission wavelength maximum is 697 nm when intercalated with dsDNA. The fluorescence signal is proportional and stoichiometric to the quantity of nuclear DNA present in the cell.

    Alternate Names

    DRAQ5

    Additional DRAQ5 (TM) Products

    Product Documents for DRAQ5 (TM)

    Certificate of Analysis

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    Product Specific Notices for DRAQ5 (TM)

    DRAQ5 (TM) is a registered trademark of BioStatus Limited.

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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    Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.