DRAQ7 (TM)

Novus Biologicals | Catalog # NBP2-81126

Novus Biologicals
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Key Product Details

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Microscopy

Product Summary for DRAQ7 (TM)

Key Features of DRAQ7 (TM) :
  • Far-red viability dye used for investigating dead or membrane compromised cells, notably for dead cell exclusion in flow cytometry, single-cell RNAseq and GWAS
  • Does not enter intact, live cells and acts as an ideal replacement for propidium iodide (PI) and 7-AAD
  • Serves as a component of apoptosis assays, for any fluorescence-based cell analysis platform, and in cell health / in vitro toxicity assays
  • Rapid staining and easy to use, without the need for a wash step
  • Non-toxic and optimal for long-term imaging studies, up to several days
  • Minimal photobleaching
  • Spectrally compatible with GFP and FITC labels.


    • DRAQ7 (TM) is supplied as a blue aqueous solution and shipped at ambient temperature, but on receipt packs should be stored at 2-8C. DO NOT FREEZE.

    Product Specifications

    Applications

    Flow Cytometry (1:100)
    Immunocytochemistry/ Immunofluorescence (1:60)
    Live Imaging Microscopy (1:100)
    Fluorescence Imaging (1:60 - 1:100)

    Application Notes

    DRAQ7 (TM) is supplied at a concentration of 0.3mM: the 250 ul size allows for 50 Flow Cytometry assays and 250 Cell Health assays whereas the 1ml size allows for 200 Flow Cytometry assays and 1,000 Cell Health assays. DRAQ7 (TM) can be diluted in culture media (e.g. RPMI 1640) and physiological buffers (eg PBS, Hankss, etc.) and mixed with fixatives such as formaldehyde. DRAQ7 (TM) has many applications in imaging, cytometry and screening and is highly compatible with existing protocols across a wide range of instrumentation platforms.

    Spectra Viewer

    Plan Your Experiments

    Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

    Spectra Viewer
    Spectra Viewer

    Scientific Data Images for DRAQ7 (TM)

    Flow Cytometry: DRAQ7 (TM) [NBP2-81126]

    Flow Cytometry: DRAQ7 (TM) [NBP2-81126]

    Flow Cytometry: DRAQ7 (TM) [NBP2-81126] - Lymphoma cells treated with increasing quantities of staurosporine (STS). reports STS-induced apoptosis and cell death in dose-dependent manner with clear separation of positive and negative events.
    Live Imaging Microscopy: DRAQ7 (TM) [NBP2-81126]

    Live Imaging Microscopy: DRAQ7 (TM) [NBP2-81126]

    Live Imaging Microscopy: DRAQ7 (TM) [NBP2-81126] - DRAQ7(TM) was added directly to unfixed THP-1 cells in RPMI + 20% FBS culture media at 3 uM (1:100) for 30 minutes at room temperature and protected from light. Imaging was done immediately after staining without washing the cells.
    Immunocytochemistry/ Immunofluorescence: DRAQ7 (TM) [NBP2-81126]

    Immunocytochemistry/ Immunofluorescence: DRAQ7 (TM) [NBP2-81126]

    Immunocytochemistry/Immunofluorescence: DRAQ7 (TM) [NBP2-81126] - Formaldehyde-fixed U2OS cells labelled with (red, nuclei+D8 and AlexaFluor 488 antibody to beta-tubulin (green).
    DRAQ7 (TM) [NBP2-81126] - Spectral properties of DRAQ7 (TM) - spectral compatibility with UV-excited and most vis. Range fluorochromes for multi-colour analysis. Detection from blue excitation is achievable only by flow cytometry.
    Fluorescence Imaging: DRAQ7 (TM) [NBP2-81126] - montage.
    DRAQ7 (TM)

    DRAQ7 (TM) [NBP2-81126] -

    DRAQ7 (TM) [NBP2-81126] - In vitro, Cortical neurons are used to assess the glutamate toxicity using DRAQ7 dye. Image from verified customer review.

    Formulation, Preparation, and Storage

    Purification

    >97%

    Concentration

    Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

    Shipping

    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

    Storage

    Store at 4C in the dark. Do not freeze.

    Background: DRAQ7 (TM)

    DRAQ7 (TM) is a far-red viability dye belonging to the anthraquinone family that is closely related to DRAQ5 (TM). DRAQ7 (TM) only stains nuclei in dead and membrane-compromised (permeabilized) cells.

    The excitation wavelength maxima of DRAQ7 (TM) are 600 nm / 646 nm but it can also be suboptimally excited by the 488nm (blue) laser for flow cytometry. The emission wavelength maximum of this viability dye is 697 nm when bound to dsDNA. The fluorescence signal is proportional and stoichiometric to the amount of bound DNA.

    DRAQ7 (TM) is a registered trademark of BioStatus Limited.

    Additional DRAQ7 (TM) Products

    Product Documents for DRAQ7 (TM)

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

    Product Specific Notices for DRAQ7 (TM)

    DRAQ7 (TM) is a registered trademark of BioStatus Limited.

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

    Customer Reviews for DRAQ7 (TM) (2)

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    Customer Images

    Showing  1 - 2 of 2 reviews Showing All
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    • DRAQ7 (TM)
      Name: Shashirekha Markandaiah
      Verified Customer | Posted 06/06/2023
      Assessing glutamate toxicity at 250 micromolar in vitro.
      In vitro, Cortical neurons are used to assess the glutamate toxicity using DRAQ7 dye.
      DRAQ7 (TM) NBP2-81126
    • DRAQ7 (TM)
      Name: Anonymous
      Verified Customer | Posted 10/12/2021
      Detects cell death very well. Neurons transfected with Tdtomato and undergoing stress for 72 hours. Draq7 labeling is evident at 72 hours.
      used at 4 micro litre per well of a 24 well dish
      DRAQ7 (TM) NBP2-81126

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    Protocols

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