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Key Product Details
Species Reactivity
Human, Mouse, Rat, Chicken
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Draxin/C1orf187 synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human Draxin/C1orf187. This peptide sequence is highly conserved in rat, mouse, and chick Draxin/C1orf187. [UniProt# Q8NBI3]
Specificity
The antibody detects a significant 50 kDa* protein corresponding to the molecular mass of Draxin/C1orf187 on SDS-PAGE immunoblots of rat PC12 cells, rat P1 brain, and chick E9 brain. The antibody also shows low expression of draxin in adult mouse brain.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Draxin/C1orf187 Antibody
Western Blot: Draxin/C1orf187 Antibody [NBP3-23287] -
Western Blot: Draxin/C1orf187 Antibody [NBP3-23287] - Western blot analysis of Draxin/C1orf187 expression in rat PC12 cells (lane 1), rat P1 brain (lane 2), adult mouse brain (lane 3), and chick E9 brain (lane 4). The blot was probed with rabbit polyclonal anti-Draxin/C1orf187 (C-terminal region) at 1:1000.
Immunocytochemistry/Immunofluorescence: Draxin/C1orf187 Antibody [NBP3-23287] -
Immunocytochemistry/Immunofluorescence: Draxin/C1orf187 Antibody [NBP3-23287] - Immunocytochemical labeling of Draxin/C1orf187 in rat PC12 cells differentiated with NGF. The cells were probed with Draxin/C1orf187 (C-terminal region) rabbit polyclonal antibody, then the antibody was detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide. Lower images show corresponding phase images.Applications for Draxin/C1orf187 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Draxin
Long Name
Dorsal Repulsive Axon Guidance Protein
Alternate Names
C1orf187, Neucrin
Gene Symbol
DRAXIN
Additional Draxin Products
Product Documents for Draxin/C1orf187 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Draxin/C1orf187 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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