Drosha Antibody (4R0B7)

Name: Drosha Antibody (4R0B7)
Brand: Novus Biologicals
SKU: NBP3-15804
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15804

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4R0B7 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DROSHA (Q9NRR4). MMQGNTCHRMSFHPGRGCPRGRGGHGARPSAPSFRPQNLRLLHPQQPPVQYQYEPPSAPSTTFSNSPAPNFLPPRPDFVPFPPPMPPSAQGPLPPCPIRP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Drosha Antibody (4R0B7)

Western Blot: Drosha Antibody (4R0B7) [NBP3-15804]

Western Blot: Drosha Antibody (4R0B7) [NBP3-15804] - Western blot analysis of extracts of various cell lines, using Drosha antibody (NBP3-15804) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunocytochemistry/ Immunofluorescence: Drosha Antibody (4R0B7) [NBP3-15804] -

Immunocytochemistry/ Immunofluorescence: Drosha Antibody (4R0B7) [NBP3-15804] - Immunofluorescence analysis of HeLa cells using Drosha antibody. Blue: DAPI for nuclear staining.

Applications for Drosha Antibody (4R0B7)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Drosha

Members of the ribonuclease III superfamily of double-stranded (ds) RNA-specific endoribonucleases participate in diverse RNA maturation and decay pathways in eukaryotic and prokaryotic cells (Fortin et al., 2002 [PubMed 12191433]). The RNase III Drosha is the core nuclease that executes the initiation step of microRNA (miRNA) processing in the nucleus (Lee et al., 2003 [PubMed 14508493]).[supplied by OMIM]

Alternate Names

drosha, double-stranded RNA-specific endoribonuclease, drosha, ribonuclease type III, EC 3.1.26.3, ETOHI2, nuclear RNase III Drosha, p241, Protein Drosha, putative protein p241 which interacts with transcription factor Sp1, putative ribonuclease III, RANSE3L, ribonuclease 3, Ribonuclease III, ribonuclease III, nuclear, RN3nuclear, RNase III

Gene Symbol

DROSHA

Additional Drosha Products

Product Documents for Drosha Antibody (4R0B7)

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Certificate of Analysis

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Product Specific Notices for Drosha Antibody (4R0B7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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