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dsRNA Antibody (K2)

Novus Biologicals | Catalog # NBP3-11398

Novus Biologicals
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Key Product Details

Species Reactivity

Multi-Species

Applications

ELISA, Sandwich ELISA, Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # K2
View all dsRNA Primary Antibodies »

Product Specifications

Immunogen

Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clone.

Specificity

This antibody recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp dsRNA. Recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (40-50 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognised by this antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Description

After delivery antibodies should be aliquoted and stored at -20 or -70C. After adding 10 mM sodium azide undiluted antibody can also be stored at 4C for a short period of time. For long term storage the mAb should be kept frozen. Repeated freezing/thawing cycles should be avoided. This ds-RNA antibody was manufactured by Scicon.

Applications for dsRNA Antibody (K2)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is primarily used for a sandwich ELISA to detect and quantitate (after calibration) dsRNA (see Schonborn et al.). For this application it should be diluted 1:2 with PBS. It may also be advantageous to use it for immunofluorescence studies. The optimum working dilution of the antibody for any specific application should be established by titration.

Not for use for clinical purposes. For in vitro use only.

dsRNA-immunoblotting.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Culture supernatant (RPMI, 5% fetal calf serum)

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: dsRNA

Double-stranded RNA (dsRNA)antibodies have been used extensively to detect and characterise plant and animal viruses with dsRNA genomes or intermediates. K2 is mainly used for specialised applications, where J2 and K1 - antibodies that can be shipped at ambient temperature - do not suffice. Due to its IgM isotype K2 is more prone to aggregation at high concentration, and therefore cannot be lyophilised and needs to be shipped on ice. Generally, K2 can be used in applications where an anti-dsRNA antibody with an isotype other than IgG (IgG2a) is required.

Alternate Names

Double-stranded RNA

Additional dsRNA Products

Product Documents for dsRNA Antibody (K2)

Certificate of Analysis

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Product Specific Notices for dsRNA Antibody (K2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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