Dynamin 3 Antibody

Western Blot: Dynamin 3 Antibody [NB120-3458]

Western Blot: Dynamin 3 Antibody [NB120-3458] - Analysis of rat liver extract.

Immunocytochemistry/ Immunofluorescence: Dynamin 3 Antibody [NB120-3458]

Immunocytochemistry/Immunofluorescence: Dynamin 3 Antibody [NB120-3458] - Immunofluorescence analysis of Dynamin III was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(TM) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Dynamin III Rabbit Polyclonal Antibody at 2 ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(TM) Secondary Antibody, Alexa Fluor(R) 488 conjugate for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.