E-Cadherin [p Ser838, p Ser840] Antibody

Novus Biologicals | Catalog # NBP3-12922

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Canine (100%), Porcine (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all E-Cadherin Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser838/Ser840 of human E-Cadherin. The exact sequence is proprietary.

Modification

p Ser838, p Ser840

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for E-Cadherin [p Ser838, p Ser840] Antibody

Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922]

Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922]

Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody (NBP3-12922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922]

Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922]

Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922] - E-Cadherin (phospho Ser838/840) antibody detects E-Cadherin (phospho Ser838/840) protein by western blot analysis. MCF-7 whole cell extract (30 ug) with the indicated treatments were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody (NBP3-12922) at a dilution of 1:10000.
Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922]

Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922]

Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922] - E-Cadherin (phospho Ser838/840) antibody detects E-Cadherin (phospho Ser838/840) protein by western blot analysis. Rat tissue extracts (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody (NBP3-12922) diluted at 1:10000.
Immunoprecipitation: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922]

Immunoprecipitation: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922]

Immunoprecipitation: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922] - Immunoprecipitation of E-Cadherin (phospho Ser838/840) protein from MCF-7 whole cell extracts using 5 ug of E-Cadherin (phospho Ser838/840) antibody (NBP3-12922). Western blot analysis was performed using E-Cadherin (phospho Ser838/840) antibody (NBP3-12922). EasyBlot anti-Rabbit IgG was used as a secondary reagent.
E-Cadherin [p Ser838, p Ser840] Antibody

Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922] -

Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody (NBP3-12922) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
E-Cadherin [p Ser838, p Ser840] Antibody

Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922] -

Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody (NBP3-12922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
E-Cadherin [p Ser838, p Ser840] Antibody

Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922] -

Untreated (-) and treated (+) mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody (NBP3-12922) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for E-Cadherin [p Ser838, p Ser840] Antibody

Application
Recommended Usage

Immunoprecipitation

1:72-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: E-Cadherin

E-cadherin, or epithelial cadherin, is an cell-cell adhesion glycoprotein important to cellular processes such as morphology, polarity, development, tissue integrity and migration. E cadherin's extracellular domain interacts with other E-cadherin protein on adjacent epithelial cells to establish adhesion between them. E cadherin has also been identified as a tumor and metastasis suppressor, and its mutations have been linked to various forms of cancer.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Gene Symbol

CDH1

Additional E-Cadherin Products

Product Documents for E-Cadherin [p Ser838, p Ser840] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for E-Cadherin [p Ser838, p Ser840] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail