E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-08913

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-UBE3A-1A2

Format

Azide and BSA Free
View all E6AP/UBE3A Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human E6AP/UBE3A protein (Uniprot: Q05086)

Localization

Nucleus, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07170).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) - Azide and BSA Free [NBP3-08913]

Immunocytochemistry/ Immunofluorescence: E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) - Azide and BSA Free [NBP3-08913]

Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) [NBP3-08913] - Immunofluorescent Analysis of PFA-fixed HeLa cells. E6AP/UBE3A Mouse Monoclonal Antibody (PCRP-UBE3A-1A2) followed by IgG-CF488 (green), counterstained with phalloidin.
Flow Cytometry: E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) - Azide and BSA Free [NBP3-08913]

Flow Cytometry: E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) - Azide and BSA Free [NBP3-08913]

Flow Cytometry: E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) [NBP3-08913] - Flow Cytometric Analysis of PFA-fixed HeLa cells. E6AP/UBE3A Mouse Monoclonal Antibody (PCRP-UBE3A-1A2) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).
Protein Array: E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) - Azide and BSA Free [NBP3-08913]

Protein Array: E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) - Azide and BSA Free [NBP3-08913]

Protein Array: E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) [NBP3-08913] - Analysis of Protein Array containing more than 19,000 full-length human proteins using E6AP/UBE3A Mouse Monoclonal Antibody (PCRP-UBE3A-1A2).

Applications for E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: E6AP/UBE3A

UBE3A encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.

Long Name

Human Papillomavirus E6-associated Protein

Alternate Names

ANCR, EPVE6AP, HPVE6A, UBE3A

Gene Symbol

UBE3A

Additional E6AP/UBE3A Products

Product Documents for E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for E6AP/UBE3A Antibody (PCRP-UBE3A-1A2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases

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Protocols

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail