EBF-3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16254

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%), Xenopus (95%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EBF-3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human EBF-3. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (95%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit EBF-3 Antibody - BSA Free (NBP2-16254) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for EBF-3 Antibody - BSA Free

Western Blot: EBF-3 Antibody [NBP2-16254]

Western Blot: EBF-3 Antibody [NBP2-16254]

Western Blot: EBF3 Antibody [NBP2-16254] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: EBF-3 Antibody [NBP2-16254]

Immunocytochemistry/ Immunofluorescence: EBF-3 Antibody [NBP2-16254]

Immunocytochemistry/Immunofluorescence: EBF-3 Antibody [NBP2-16254] - U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EBF3 protein stained by EBF3 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um
Immunoprecipitation: EBF-3 Antibody [NBP2-16254]

Immunoprecipitation: EBF-3 Antibody [NBP2-16254]

Immunoprecipitation: EBF-3 Antibody [NBP2-16254] - Immunoprecipitation of EBF3 protein from U87-MG whole cell extracts using 5 ug of EBF3 antibody [C1C3] Western blot analysis was performed using EBF3 antibody [C1C3] Easy Blot anti-Rabbit IgG was used as a secondary reagent.

Applications for EBF-3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EBF-3

Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.

Long Name

Early B-cell Factor 3

Alternate Names

COE3, EBF3, OE-2

Gene Symbol

EBF3

UniProt

Q9H4W6 (Human)

Additional EBF-3 Products

Product Documents for EBF-3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for EBF-3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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