EBI3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-48852

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EBI3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SPVSFIATYRLGMAARGHSWPCLQQTPTSTSCTITDVQLFSMAPYVLNVTAVHPWGSSSSFVPFITEHIIKPDPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSLKYWIRYKRQGAARFHRVGPIEATSFILRAV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit EBI3 Antibody - BSA Free (NBP2-48852) is a polyclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for EBI3 Antibody - BSA Free

Immunohistochemistry-Paraffin: EBI3 Antibody [NBP2-48852]

Immunohistochemistry-Paraffin: EBI3 Antibody [NBP2-48852]

Immunohistochemistry-Paraffin: IL-27/IL-35 EBI3 Subunit Antibody [NBP2-48852] - Staining in human placenta and prostate tissues using anti-EBI3 antibody. Corresponding EBI3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: EBI3 Antibody [NBP2-48852]

Immunohistochemistry-Paraffin: EBI3 Antibody [NBP2-48852]

Immunohistochemistry-Paraffin: IL-27/IL-35 EBI3 Subunit Antibody [NBP2-48852] - Staining of human prostate shows low expression as expected.
EBI3 Antibody

Immunohistochemistry-Paraffin: IL-27/IL-35 EBI3 Subunit Antibody [NBP2-48852] -

Immunohistochemistry-Paraffin: IL-27/IL-35 EBI3 Subunit Antibody [NBP2-48852] - Analysis in human placenta and prostate tissues using antibody. Corresponding EBI3 RNA-seq data are presented for the same tissues.
EBI3 Antibody

Immunohistochemistry-Paraffin: IL-27/IL-35 EBI3 Subunit Antibody [NBP2-48852] -

Immunohistochemistry-Paraffin: IL-27/IL-35 EBI3 Subunit Antibody [NBP2-48852] -Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
EBI3 Antibody

Immunohistochemistry-Paraffin: IL-27/IL-35 EBI3 Subunit Antibody [NBP2-48852] -

Immunohistochemistry-Paraffin: IL-27/IL-35 EBI3 Subunit Antibody [NBP2-48852] -Staining of human duodenum shows moderate cytoplasmic positivity in goblet cells.
EBI3 Antibody

Immunohistochemistry-Paraffin: IL-27/IL-35 EBI3 Subunit Antibody [NBP2-48852] -

Immunohistochemistry-Paraffin: IL-27/IL-35 EBI3 Subunit Antibody [NBP2-48852] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.

Applications for EBI3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EBI3

EBI3 gene encodes a 229 amino acid long, 25 kDA interleukin-27 subunit beta protein that is member to the hematopoitetin receptor family. These proteins obtain pro as well as anti-inflammatory properties and have roles in T-helper cell development, suppressing cell proliferation, encouraging cytotoxic T-cell activity, and can induce isotope switching in B cells. EBI3 participates in Th1 differentiation, IL-2 signaling (and its primary biological effects in various immune cells), as well as IL27-mediated signaling events. EBI3 interacts with genes IL27, IL12A, CANX, MDF1, and SMAD3. It has been associated and researched in sepsis, asthma, carcinoma, Chron's disease, b-cell lymphomas, ulcerative colitis, Hodgkin disease, lymphoma, ebv infections, and glomerulonephritis.

Long Name

Epstein-Barr virus induced gene 3

Alternate Names

EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3

Gene Symbol

EBI3

Additional EBI3 Products

Product Documents for EBI3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for EBI3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Regulatory T Cells (Tregs)

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Protocols

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