EBV Latent Membrane Protein 1 Antibody (LMPO24) - BSA Free
Novus Biologicals | Catalog # NBP2-50383
![Flow (Intracellular): EBV Latent Membrane Protein 1 Antibody (LMPO24) - BSA Free [NBP2-50383]](https://resources.rndsystems.com/images/products/EBV-Latent-Membrane-Protein-1-Antibody-LMPO24-Flow-Intracellular-NBP2-50383-img0001.jpg "Flow (Intracellular): EBV Latent Membrane Protein 1 Antibody (LMPO24) - BSA Free [NBP2-50383]")
Key Product Details
Species Reactivity
Validated:
Virus
Cited:
Virus
Applications
Validated:
Immunohistochemistry, Flow Cytometry, Flow (Intracellular), CyTOF-ready
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # LMPO24
Format
BSA Free
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Product Specifications
Immunogen
Purified plasma membranes from EBV Latent Membrane Protein 1 expressing insect cells infected with a recombinant EBV Latent Membrane Protein 1 baculovirus
Specificity
Niedobitek et al., 1995 (PMID: 7605996): "The LMPO24 clone demonstrates strong and specific reactivity in immunohistochemical staining of paraffin-embedded tissues."
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for EBV Latent Membrane Protein 1 Antibody (LMPO24) - BSA Free
Flow (Intracellular): EBV Latent Membrane Protein 1 Antibody (LMPO24) - BSA Free [NBP2-50383]
Flow (Intracellular): EBV Latent Membrane Protein 1 Antibody (LMPO24) [NBP2-50383] - Intracellular staining of LMP1 using LMPO24 antibody. NK and T cell lines were fixed and permeabilised with an EBiosciences kit (Cat 88-8824-00) then stained with LMPO24 monoclonal antibody, followed by detection with Alexa Fluor 488 conjugated anti-mouse IgG antibody and analysis by flow cytometry. The histograms show negative control fluorescence staining (black lines) and LMPO24 staining (red lines). MEC04, SNK1, SNK6 and SNT8 are EBV positive tumour cell lines derived from patients with nasal NK/T cell lymphomas. MEC04, SNK1 and SNK6 are NK cell lines, while SNT8 is a T cell line. MEC04 lacks LMP1 expression, SNK1 expresses weak to moderate levels of LMP1, whilst SNK6 and SNT8 express high levels of LMP1.Applications for EBV Latent Membrane Protein 1 Antibody (LMPO24) - BSA Free
Application
Recommended Usage
Flow (Intracellular)
1:10 - 1:1000
Flow Cytometry
1:10 - 1:1000
Immunohistochemistry
1:10 - 1:500
Application Notes
Positive control(s): EBV transformed lymphoblastoid cell lines. This antibody is CyTOF-ready.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Product Documents for EBV Latent Membrane Protein 1 Antibody (LMPO24) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EBV Latent Membrane Protein 1 Antibody (LMPO24) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for EBV Latent Membrane Protein 1 Antibody (LMPO24) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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