ECM-1/Secretory Component P85 Antibody (ECM1/792) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-48176

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # ECM1/792

Format

IHC-Prediluted
View all ECM1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human ECM-1/Secretory Component P85 protein (Uniprot: Q16610)

Localization

Cell surface and cytoplasmic

Specificity

This monoclonal antibody reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for ECM-1/Secretory Component P85 Antibody (ECM1/792) - IHC-Prediluted

Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (ECM1/792) - IHC-Prediluted [NBP2-48176]

Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (ECM1/792) - IHC-Prediluted [NBP2-48176]

Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (rECM1/792) - IHC-Prediluted [NBP2-48176] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with ECM-1/Secretory Component P85 Mouse Monoclonal Antibody (ECM1/792).
Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (ECM1/792) - IHC-Prediluted [NBP2-48176]

Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (ECM1/792) - IHC-Prediluted [NBP2-48176]

Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (rECM1/792) - IHC-Prediluted [NBP2-48176] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with IgA Secretory Component Mouse Monoclonal Antibody (ECM1/792).

Applications for ECM-1/Secretory Component P85 Antibody (ECM1/792) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistology (Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ECM1

Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.

Long Name

Extracellular Matrix Protein 1

Alternate Names

ECM1, p85, Secretory Component P85

Gene Symbol

ECM1

Additional ECM1 Products

Product Documents for ECM-1/Secretory Component P85 Antibody (ECM1/792) - IHC-Prediluted

Certificate of Analysis

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Product Specific Notices for ECM-1/Secretory Component P85 Antibody (ECM1/792) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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