ECSIT Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92814

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ECSIT Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 50-296 of human ECSIT (NP_001135936.1). SEQSLVPSPPEPRQRPTKALVPFEDLFGQAPGGERDKASFLQTVQKFAEHSVRKRGHIDFIYLALRKMREYGVERDLAVYNQLLNIFPKEVFRPRNIIQRIFVHYPRQQECGIAVLEQMENHGVMPNKETEFLLIQIFGRKSYPMLKLVRLKLWFPRFMNVNPFPVPRDLPQDPVELAMFGLRHMEPDLSARVTIYQVPLPKDSTGAADPPQPHIVGSGRDAGGVEPLLPDAAGPGVCEEWLGQLRV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ECSIT Antibody - BSA Free

ECSIT Antibody - BSA Free

Western Blot: ECSIT Antibody - BSA Free [NBP2-92814] -

Western Blot: ECSIT Antibody - BSA Free [NBP2-92814] - Western blot analysis of extracts of HepG2 cells, using ECSIT antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
ECSIT Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ECSIT Antibody - BSA Free [NBP2-92814] -

Immunocytochemistry/ Immunofluorescence: ECSIT Antibody - BSA Free [NBP2-92814] - Immunofluorescence analysis of L929 cells using ECSIT Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
ECSIT Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ECSIT Antibody - BSA Free [NBP2-92814] -

Immunocytochemistry/ Immunofluorescence: ECSIT Antibody - BSA Free [NBP2-92814] - Immunofluorescence analysis of U2OS cells using ECSIT Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
ECSIT Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ECSIT Antibody - BSA Free [NBP2-92814] -

Immunocytochemistry/ Immunofluorescence: ECSIT Antibody - BSA Free [NBP2-92814] - Immunofluorescence analysis of H9C2 cells using ECSIT Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Applications for ECSIT Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ECSIT

Activation of NF-kB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2) to kinases such as IRAK (3) and other intermediates such as TNF receptor associated factor (TRAF)-6 (4). ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway (5). Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis (6), indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

Alternate Names

ECSIT homolog (Drosophila), evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, likely ortholog of mouse signaling intermediate in Toll pathway evolutionarilyconserved, Protein SITPEC, signaling intermediate in Toll pathway evolutionarily conserved ortholog, SITPECsignaling intermediate in Toll pathway, evolutionarily conserved

Gene Symbol

ECSIT

Additional ECSIT Products

Product Documents for ECSIT Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ECSIT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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