EDNRB/Endothelin R Type B Antibody - Azide and BSA Free

Western Blot: EDNRB/Endothelin R Type B Antibody - Azide and BSA Free [NBP1-30599] -

EDNRB overexpression promotes thermogenic differentiation of human white adipocytes in vitro.a Schematic of EDNRB gene activation using CRISPR-SAM system. b–g Human white preadipocytes were transfected with the CRISPR-SAM system and either empty vector (EV) or sgRNA#3 targeting EDNRB (EDNRB OE) and were differentiated to mature adipocytes. b Relative EDNRB and EDNRA mRNA levels in human white adipocytes. n = 3 biological replicates/group. c Relative EDNRB protein levels in human white adipocytes. n = 4 biological replicates/group. d Relative mRNA expressions in adipogenic and thermogenic genes in human white adipocytes. n = 3 biological replicates/group. e Fatty acid-dependent oxygen consumption rate (OCR) in human white adipocytes. Quantification of maximal OCR in the right panel. EV: n = 3, EDNRB OE: n = 4, EV-ETO: n = 4, EDNRB OE-ETO: n = 3 biological replicates. Pal, palmitate-BSA. OA, oligomycin A. AA, antimycin A. ETO, etomoxir. f Forskolin (FSK)-stimulated OCR in human white adipocytes. n = 5 biological replicates/group. g Thermogenesis capacity monitored by ERthermAC dye in human white adipocytes. Area above the curve (AAC) of thermogenesis in the right panel. EV: n = 21 cells, EDNRB OE: n = 22 cells. Data are presented as mean +/- SEM. P values were determined using unpaired two-tailed t tests: (b–g). Source data are provided as a Source Data file. Panel (a) was created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39174539), licensed under a CC-BY license. Not internally tested by Novus Biologicals.