EEN Antibody (2A9H4) - BSA Free

Novus Biologicals | Catalog # NBP2-52560

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2A9H4

Format

BSA Free
View all EEN Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human EEN (AA: 12-119) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

41.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EEN Antibody (2A9H4) - BSA Free

Western Blot: EEN Antibody (2A9H4)BSA Free [NBP2-52560]

Western Blot: EEN Antibody (2A9H4)BSA Free [NBP2-52560]

Western Blot: EEN Antibody (2A9H4) [NBP2-52560] - Analysis using SH3GL1 mAb against human SH3GL1 (AA: 12-119) recombinant protein. (Expected MW is 37.6 kDa)
Flow Cytometry: EEN Antibody (2A9H4) - BSA Free [NBP2-52560]

Flow Cytometry: EEN Antibody (2A9H4) - BSA Free [NBP2-52560]

Flow Cytometry: EEN Antibody (2A9H4) [NBP2-52560] - Analysis of Hela cells using SH3GL1 mouse mAb (green) and negative control (red).
Western Blot: EEN Antibody (2A9H4)BSA Free [NBP2-52560]

Western Blot: EEN Antibody (2A9H4)BSA Free [NBP2-52560]

Western Blot: EEN Antibody (2A9H4) [NBP2-52560] - Analysis using SH3GL1 mAb against HEK293 (1) and SH3GL1 (AA: 12-119)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: EEN Antibody (2A9H4) - BSA Free [NBP2-52560]

ELISA: EEN Antibody (2A9H4) - BSA Free [NBP2-52560]

ELISA: EEN Antibody (2A9H4) [NBP2-52560] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).

Applications for EEN Antibody (2A9H4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EEN

Endophilin A2 is an SH3 domain containing protein implicated in endocytosis. It has been identified in human acute leukemia as a fusion partner of MLL (mixed-lineage leukemia). Because of its involvement in this chromosomal translocation, endophilin A2 is also known as EEN (extra eleven nineteen).

Alternate Names

CNSA1endophilin-2, EEN fusion partner of MLL, EENSH3 domain-containing GRB2-like protein 1, Endophilin-2, extra 11-19 leukemia fusion, Extra eleven-nineteen leukemia fusion gene protein, MGC111371, SH3 domain protein 2B, SH3-containing Grb-2-like 1 protein, SH3D2Bendophilin-A2, SH3-domain GRB2-like 1, SH3P8

Gene Symbol

SH3GL1

Additional EEN Products

Product Documents for EEN Antibody (2A9H4) - BSA Free

Certificate of Analysis

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Product Specific Notices for EEN Antibody (2A9H4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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