EFHA1 Antibody (PSH01-84)

Western Blot: EFHA1 Antibody (PSH01-84) [NBP3-32595]

Western Blot: EFHA1 Antibody (PSH01-84) [NBP3-32595] - Western blot analysis of EFHA1 on different lysates with Rabbit anti-EFHA1 antibody (NBP3-32595) at 1/1,000 dilution.

Lane 1: LNCaP cell lysate, 30 ug/Lane.
Lane 2: NIH/3T3 cell lysate, 30 ug/Lane.
Lane 3: PC-12 cell lysate, 30 ug/Lane.
Lane 4: Human kidney tissue lysate, 30 ug/Lane.
Lane 5: Human liver tissue lysate, 30 ug/Lane.
Lane 6: Mouse heart tissue lysate, 30 ug/Lane.
Lane 7: Mouse kidney tissue lysate, 30 ug/Lane.
Lane 8: Rat kidney tissue lysate, 30 ug/Lane.

Predicted band size: 50 kDa
Observed band size: 50 kDa

Exposure time: 42 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32595) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: EFHA1 Antibody (PSH01-84) [NBP3-32595]

Immunohistochemistry: EFHA1 Antibody (PSH01-84) [NBP3-32595] - Immunofluorescence analysis of paraffin-embedded human heart tissue labeling EFHA1 with Rabbit anti-EFHA1 antibody (NBP3-32595) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32595, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: EFHA1 Antibody (PSH01-84) [NBP3-32595]

Immunohistochemistry: EFHA1 Antibody (PSH01-84) [NBP3-32595] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-EFHA1 antibody (NBP3-32595) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32595) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.