EG-VEGF/PK1 Antibody (1B4G2)

Novus Biologicals | Catalog # NBP3-15678

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1B4G2 expressed in HEK293
View all EG-VEGF/PK1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human EG-VEGF/PK1 (P58294). MRGATRVSIMLLLVTVSDCAVITGACERDVQCGAGTCCAISLWLRGLRMCTPLGREGEECHPGSHKVPFFRKRKHHTCPCLPNLLCSRFPDGRYRCSMDLKNINF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EG-VEGF/PK1 Antibody (1B4G2)

EG-VEGF/PK1 Antibody (1B4G2)

Western Blot: EG-VEGF/PK1 Antibody (1B4G2) [EG-VEGF/PK1] -

Western Blot: EG-VEGF/PK1 Antibody (1B4G2) [EG-VEGF/PK1] - Western blot analysis of various lysates using PROK1 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
EG-VEGF/PK1 Antibody (1B4G2)

Immunocytochemistry/ Immunofluorescence: EG-VEGF/PK1 Antibody (1B4G2) [EG-VEGF/PK1] -

Immunocytochemistry/ Immunofluorescence: EG-VEGF/PK1 Antibody (1B4G2) [EG-VEGF/PK1] - Immunofluorescence analysis of paraffin-embedded human placenta using PROK1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
EG-VEGF/PK1 Antibody (1B4G2)

Immunocytochemistry/ Immunofluorescence: EG-VEGF/PK1 Antibody (1B4G2) [EG-VEGF/PK1] -

Immunocytochemistry/ Immunofluorescence: EG-VEGF/PK1 Antibody (1B4G2) [EG-VEGF/PK1] - Immunofluorescence analysis of paraffin-embedded rat placenta using PROK1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for EG-VEGF/PK1 Antibody (1B4G2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EG-VEGF/PK1

Prokineticin 1 potently contracts gastrointestinal (GI) smooth muscle. It induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands and has little or no effect on a variety of other endothelial and non-endothelial cell types.

Long Name

Endocrine Gland-derived Vascular Endothelial Growth Factor

Alternate Names

EGVEGF, PROK1, Prokineticin 1

Gene Symbol

PROK1

Additional EG-VEGF/PK1 Products

Product Documents for EG-VEGF/PK1 Antibody (1B4G2)

Certificate of Analysis

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Product Specific Notices for EG-VEGF/PK1 Antibody (1B4G2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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