![Immunohistochemistry-Paraffin: EGR4 Antibody [NBP1-80999]](https://resources.rndsystems.com/images/products/EGR4-Antibody-Immunohistochemistry-Paraffin-NBP1-80999-img0006.jpg "Immunohistochemistry-Paraffin: EGR4 Antibody [NBP1-80999]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PWELLSVGAPGNCGSQGDYQAAPEARFPVIGTKIEDLLSISCPAELPAVPANRLYPSGAYDAFPLAPGDLGEGAEGLPGLLTPPSGEGGSSGDGGEFLASTQPQLSPLGLRSAAAADFPKPL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for EGR4 Antibody - BSA Free
Immunohistochemistry-Paraffin: EGR4 Antibody [NBP1-80999]
Immunohistochemistry-Paraffin: EGR4 Antibody [NBP1-80999] - Analysis in human cerebral cortex and prostate tissues using NBP1-80999 antibody. Corresponding EGR4 RNA-seq data are presented for the same tissues.![EGR4 Antibody - BSA Free Immunohistochemistry-Paraffin: EGR4 Antibody [NBP1-80999]](https://resources.rndsystems.com/images/products/antibody/nbp1-80999_rabbit-polyclonal-egr4-antibody-immunohistochemistry-paraffin-2110202513278.jpg "Immunohistochemistry-Paraffin: EGR4 Antibody [NBP1-80999]")
Immunohistochemistry-Paraffin: EGR4 Antibody [NBP1-80999]
Staining of human cerebral cortex shows moderate cytoplasmic and nuclear positivity in neuronal and glial cells.![EGR4 Antibody - BSA Free Immunohistochemistry-Paraffin: EGR4 Antibody [NBP1-80999]](https://resources.rndsystems.com/images/products/antibody/nbp1-80999_rabbit-polyclonal-egr4-antibody-immunohistochemistry-paraffin-2110202513284.jpg "Immunohistochemistry-Paraffin: EGR4 Antibody [NBP1-80999]")
Immunohistochemistry-Paraffin: EGR4 Antibody [NBP1-80999]
Staining of human prostate shows no nuclear positivity in glandular cells.![EGR4 Antibody - BSA Free Immunohistochemistry-Paraffin: EGR4 Antibody [NBP1-80999]](https://resources.rndsystems.com/images/products/antibody/nbp1-80999_rabbit-polyclonal-egr4-antibody-immunohistochemistry-paraffin-21102025132921.jpg "Immunohistochemistry-Paraffin: EGR4 Antibody [NBP1-80999]")
Immunohistochemistry-Paraffin: EGR4 Antibody [NBP1-80999]
Staining of human pancreas shows no nuclear positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: EGR4 Antibody [NBP1-80999
Staining of human colon shows no positivity in glandular cells as expected.
Chromatin Immunoprecipitation ChIP: EGR4 Antibody - BSA Free
ChIP-Exo-Seq composite graph for Anti-EGR4 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.Applications for EGR4 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EGR4
Alternate Names
AT133, early growth response 4, early growth response protein 4, EGR-4, NGFIC, NGFI-C, PAT133
Entrez Gene IDs
1961 (Human)
Gene Symbol
EGR4
Additional EGR4 Products
Product Documents for EGR4 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EGR4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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