![Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-33665]](https://resources.rndsystems.com/images/products/EHD4-Antibody-Immunohistochemistry-Paraffin-NBP2-33665-img0004.jpg "Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-33665]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: FGKENKKRELISRLPEIYIQLQREYQISAGDFPEVKAMQEQLENYDFTKFH
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for EHD4 Antibody - BSA Free
Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-33665]
Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-33665] - Staining of human fallopian tube shows strong positivity in endothelial cells.![Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-33665]](https://resources.rndsystems.com/images/products/EHD4-Antibody-Immunohistochemistry-Paraffin-NBP2-33665-img0002.jpg "Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-33665]")
Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-33665]
Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-33665] - Staining of human urinary bladder shows moderate membranous and cytoplasmic positivity in urothelial cells, as well as in endothelial cells.![Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-33665]](https://resources.rndsystems.com/images/products/EHD4-Antibody-Immunohistochemistry-Paraffin-NBP2-33665-img0003.jpg "Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-33665]")
Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-33665]
Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-33665] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.![Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-33665]](https://resources.rndsystems.com/images/products/EHD4-Antibody-Immunohistochemistry-Paraffin-NBP2-33665-img0005.jpg "Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-33665]")
Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-33665]
Immunohistochemistry-Paraffin: EHD4 Antibody [NBP2-33665] - Staining of human duodenum shows strong positivity in endothelial cells.Applications for EHD4 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EHD4
Alternate Names
EH domain containing 4, EH domain-containing protein 4, EH-domain containing 4, HCA10, HCA11, Hepatocellular carcinoma-associated protein 10/11, hepatocellular carcinoma-associated protein HCA11, ortholog of rat pincher, PAST homolog 4, PAST4
Gene Symbol
EHD4
UniProt
Additional EHD4 Products
Product Documents for EHD4 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EHD4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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