eIF2A [p Ser51] Antibody (0011) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-23508

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # 0011 expressed in HEK293

Format

Azide and BSA Free
View all eIF2A Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Ser51 of human phospho-eIF2A.

Modification

p Ser51

Clonality

Monoclonal

Host

Rabbit

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.

Scientific Data Images for eIF2A [p Ser51] Antibody (0011) - Azide and BSA Free

eIF2A Antibody (0011)

Western Blot: eIF2A [p Ser51] Antibody (0011) [NBP3-23508] -

Western Blot: eIF2A [p Ser51] Antibody (0011) [NBP3-23508] - Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (100nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using NBP3-23508 at 1:1000 dilution.

Applications for eIF2A [p Ser51] Antibody (0011) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF2A

The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]

Alternate Names

EIF-2, EIF-2A, EIF2AEIF2, EIF-2alpha, eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa, subunit 1 (alpha, 35kD )

Gene Symbol

EIF2A

Additional eIF2A Products

Product Documents for eIF2A [p Ser51] Antibody (0011) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for eIF2A [p Ser51] Antibody (0011) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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