EIF2AK1 Antibody (1G4Z2)

Novus Biologicals | Catalog # NBP3-33479

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 1G4Z2
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 337-586 of human EIF2AK1.2).

Sequence:
SIVEQQLPLRRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLLQSELFQNS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EIF2AK1 Antibody (1G4Z2)

EIF2AK1 Antibody (1G4Z2)

Western Blot: EIF2AK1 Antibody (1G4Z2) [NBP3-33479] -

Western Blot: EIF2AK1 Antibody (1G4Z2) [NBP3-33479] - Western Blot analysis of lysates from wild type(WT) and EIF2AK1 knockdown (KD) 293T cells, using [KD Validated] EIF2AK1 Rabbit mAb at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Applications for EIF2AK1 Antibody (1G4Z2)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EIF2AK1

EIF2AK1 is encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

EC 2.7.11, eukaryotic translation initiation factor 2-alpha kinase 1, HCR, heme sensitive initiation factor 2a kinase, Heme-controlled repressor, Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase, Heme-regulated inhibitor, heme-regulated initiation factor 2-alpha kinase, heme-regulated repressor, Hemin-sensitive initiation factor 2-alpha kinase, HRIEC 2.7.11.1, KIAA1369heme regulated initiation factor 2 alpha kinase

Gene Symbol

EIF2AK1

Additional EIF2AK1 Products

Product Documents for EIF2AK1 Antibody (1G4Z2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for EIF2AK1 Antibody (1G4Z2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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