EIF5 Antibody

Name: EIF5 Antibody
Brand: Novus Biologicals
SKU: NB100-93312
Price: 459 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-93312

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human eukaryotic translation initiation factor 5 using the numbering given in entry NP_892116.2 (GeneID 1983).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EIF5 Antibody

Western Blot: EIF5 Antibody [NB100-93312]

Western Blot: EIF5 Antibody [NB100-93312] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibody used for WB at 0.04 ug/ml.

Applications for EIF5 Antibody

Application

Recommended Usage

Western Blot

1:2000-1:10000

Application Notes

This antibody is useful for western blot. It is not recommended for immunoprecipitation.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EIF5

Eukaryotic initiation factor 5 (eIF5) plays an important role in the assembly of the 43S pre-initiation complex. In this assembly, eIF5 interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 40S ribosome subunit. eIF5 is also reported to form a complex with eIF2. This complex is suggested to serve as a cytoplasmic reservoir for eIF2 that restricts the guanine nucleotide exchange promoted by the eIF2 beta subunit for the initiation of translation.

Alternate Names

eIF-5, EIF-5A, eukaryotic translation initiation factor 5

Entrez Gene IDs

1983 (Human); 217869 (Mouse)

Gene Symbol

EIF5

UniProt

NP_892116.2 (Human)

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Product Documents for EIF5 Antibody

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Product Specific Notices for EIF5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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