EMILIN1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84127

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EMILIN1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PQSIMYRRFLRPRYRVAYKTVTDMEWRCCQGYGGDDCAESPAPALGPASSTPRPLARPARPNLSGSSAGSPLSGLGGEGPGESEKVQQLEEQVQSLTKELQGLRGVLQGLSGRLAEDVQRAVETAFNGRQQPADAAARPGVHETLNE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EMILIN1 Antibody - BSA Free

Immunohistochemistry-Paraffin: EMILIN1 Antibody [NBP1-84127]

Immunohistochemistry-Paraffin: EMILIN1 Antibody [NBP1-84127]

Immunohistochemistry-Paraffin: EMILIN1 Antibody [NBP1-84127] - Staining of human prostate shows strong membranous positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: EMILIN1 Antibody [NBP1-84127]

Immunohistochemistry-Paraffin: EMILIN1 Antibody [NBP1-84127]

Immunohistochemistry-Paraffin: EMILIN1 Antibody [NBP1-84127] - Staining of human skin shows strong positivity in connective tissue.
Immunohistochemistry-Paraffin: EMILIN1 Antibody [NBP1-84127]

Immunohistochemistry-Paraffin: EMILIN1 Antibody [NBP1-84127]

Immunohistochemistry-Paraffin: EMILIN1 Antibody [NBP1-84127] - Staining of human testis shows moderate membranous positivity in peritubular myoid cells.
Immunohistochemistry-Paraffin: EMILIN1 Antibody [NBP1-84127]

Immunohistochemistry-Paraffin: EMILIN1 Antibody [NBP1-84127]

Immunohistochemistry-Paraffin: EMILIN1 Antibody [NBP1-84127] - Staining of human cerebral cortex shows no positivity in neurons as expected.
EMILIN1 Antibody - BSA Free Western Blot: EMILIN1 Antibody - BSA Free [NBP1-84127]

Western Blot: EMILIN1 Antibody - BSA Free [NBP1-84127]

Analysis in human cell line SH-SY5Y.

Applications for EMILIN1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/mL
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-84127 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EMILIN1

The EMILIN1 gene encodes an extracellular matrix glycoprotein that is characterized by an N-terminal microfibril interfacedomain, a coiled-coiled alpha-helical domain, a collagenous domain and a C-terminal globular C1q domain. The encodedprotein associates with

Alternate Names

DKFZp586M121, elastin microfibril interface-located protein 1, elastin microfibril interfacer 1, EMI, EMILIN, EMILIN-1, gp115

Gene Symbol

EMILIN1

Additional EMILIN1 Products

Product Documents for EMILIN1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for EMILIN1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for EMILIN1 Antibody - BSA Free

Customer Reviews for EMILIN1 Antibody - BSA Free (2)

4 out of 5
2 Customer Ratings
5 Stars
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4 Stars
100%
3 Stars
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2 Stars
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1 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • EMILIN1 Antibody
    Name: Catherine Heyward
    Application: Immunocytochemistry
    Sample Tested: Cell culture
    Species: Human
    Verified Customer | Posted 08/03/2021
    Human periodontal ligament cells stained with anti-emilin1 1:300 and goat anti-rabbit Alexa Fluor 488 1:500. Nuclei costained with 300nM DAPI.
    EMILIN1 Antibody - BSA Free NBP1-84127
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • EMILIN1 Antibody
    Name: Catherine Heyward
    Application: Immunohistochemistry-Paraffin
    Sample Tested: FFPE and IHC-P Sample Tested
    Species: Rat
    Verified Customer | Posted 08/03/2021
    5um section of formalin-fixed paraffin-embedded rat nasal concha stained with anti-emilin1 (1:300) and goat anti-rabbit Alexa Fluor 488 (1:500) and DAPI (300nM).
    Citrate buffer antigen retrieval 60C overnight.
    EMILIN1 Antibody - BSA Free NBP1-84127
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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FAQs

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