EMMPRIN/CD147 Antibody (23GB1400)

Novus Biologicals | Catalog # NBP3-51268

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB1400
View all EMMPRIN/CD147 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human EMMPRIN/CD147 (Uniprot: P35613)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EMMPRIN/CD147 Antibody (23GB1400)

EMMPRIN/CD147 Antibody (23GB1400)

Immunocytochemistry/ Immunofluorescence: EMMPRIN/CD147 Antibody (23GB1400)[NBP3-51268] -

EMMPRIN/CD147 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: CD147 shRNA knockdown (KD). Nuclei were stained blue with DAPI; CD147 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
EMMPRIN/CD147 Antibody (23GB1400)

Immunocytochemistry/ Immunofluorescence: EMMPRIN/CD147 Antibody (23GB1400)[NBP3-51268] -

Immunocytochemical staining of HepG2 cells with EMMPRIN/CD147 antibody (1:1,000). Nuclei were stained blue with DAPI; EMMPRIN/CD147 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: medium. Scale bar, 20 um.
EMMPRIN/CD147 Antibody (23GB1400)

Flow Cytometry: EMMPRIN/CD147 Antibody (23GB1400)[NBP3-51268] -

Validation of EMMPRIN/CD147 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with EMMPRIN/CD147 antibody (1:2,000) and analyzed using BD flow cytometer.
EMMPRIN/CD147 Antibody (23GB1400)

Flow Cytometry: EMMPRIN/CD147 Antibody (23GB1400)[NBP3-51268] -

Flow cytometric analysis of EMMPRIN/CD147expression in HepG2 cells using EMMPRIN/CD147 antibody (1:2,000). Green, isotype control; red, CD147.
EMMPRIN/CD147 Antibody (23GB1400)

Western Blot: EMMPRIN/CD147 Antibody (23GB1400)[NBP3-51268] -

Western blotting analysis using EMMPRIN/CD147 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for EMMPRIN/CD147 Antibody (23GB1400)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EMMPRIN/CD147

CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.

Long Name

Extracellular Matrix Metalloproteinase Inducer

Alternate Names

Basigin, BSG, CD147

Gene Symbol

BSG

Additional EMMPRIN/CD147 Products

Product Documents for EMMPRIN/CD147 Antibody (23GB1400)

Certificate of Analysis

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Product Specific Notices for EMMPRIN/CD147 Antibody (23GB1400)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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