Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # OX114

Format

BSA Free
View all EMMPRIN/CD147 Primary Antibodies »

Product Specifications

Immunogen

Fusion protein mCD147dO-2rCD4d3 +4

Specificity

NB100-65518 recognizes mouse CD147 (also known as basigin). CD147 is a cell surface glycoprotein that is widely expressed on leukocytes, red cells, platelets and endothelial cells. The reported functions of CD147 are diverse and include involvement in T-cell activation, cell adhesion and induction of matrix metalloproteinases. OX-114 recognizes both isoforms of CD147, the common form containing 2 Ig-like domains and the rare form containing 3 Ig-like domains. This antibody is routinely tested in flow cytometry on mouse splenocytes. Note that for staining of whole blood samples increased amounts of antibody may be required due to the presence of CD147 on erythrocytes.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for EMMPRIN/CD147 Antibody (OX114) - BSA Free

Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) - BSA Free [NB100-65518]

Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) - BSA Free [NB100-65518]

Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Analysis using the FITC conjugate of NB100-65518. Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD147: FITC.
Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) - BSA Free [NB100-65518]

Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) - BSA Free [NB100-65518]

Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Staining of mouse peripheral blood lymphocytes with RAT ANTI MOUSE CD147.
Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) - BSA Free [NB100-65518]

Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) - BSA Free [NB100-65518]

Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Staining of mouse peripheral blood lymphocytes with Rat anti MouseE CD147: FITC.
Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) - BSA Free [NB100-65518]

Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) - BSA Free [NB100-65518]

Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Staining of mouse spleen cells with Rat anti Mouse CD147: Alexa Fluor 488.
Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) - BSA Free [NB100-65518]

Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) - BSA Free [NB100-65518]

Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Analysis using the Biotin conjugate of NB100-65518. Staining of mouse spleen cells with RAT ANTI MOUSE CD147: BIOTIN.

Applications for EMMPRIN/CD147 Antibody (OX114) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:25-1:50

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EMMPRIN/CD147

CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.

Long Name

Extracellular Matrix Metalloproteinase Inducer

Alternate Names

Basigin, BSG, CD147

Gene Symbol

BSG

UniProt

P18572

Additional EMMPRIN/CD147 Products

Product Documents for EMMPRIN/CD147 Antibody (OX114) - BSA Free

Certificate of Analysis

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Product Specific Notices for EMMPRIN/CD147 Antibody (OX114) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for EMMPRIN/CD147 Antibody (OX114) - BSA Free

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Protocols

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FAQs

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