Endothelin-1 Quantikine ELISA Kit DET100: R&D Systems

Endothelin-1 Quantikine ELISA Kit

(16 citations)
(2 Reviews)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (75 uL), Serum (75 uL), EDTA Plasma (75 uL), Heparin Plasma (75 uL), Urine (75 uL)
  • Sensitivity
    0.207 pg/mL
  • Assay Range
    0.4 - 25 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
  • Specificity
    Synthetic and natural Endothelin-1
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC82, Quantikine Immunoassay Control Set 907 for Endothelin-1 - Please Inquire
Product Summary
The Quantikine Endothelin-1 immunoassay is a 4.5 hour solid phase ELISA designed to measure ET-1 in cell culture supernates, serum, plasma, and urine. It contains synthetic ET-1 and antibodies raised against synthetic ET-1. This immunoassay has been shown to accurately quantitate synthetic and naturally occurring ET-1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean37.3414.73.057.4314.4
Standard Deviation0.120.170.280.2310.4380.759
CV%42.31.97.65.95.3

Recovery

The recovery of Endothelin-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 88-108
Human EDTA Plasma (n=4) 93 87-102
Human Heparin Plasma (n=4) 93 85-106
Human Serum (n=4) 98 86-107
Mouse EDTA Plasma (n=4) 99 102-107
Mouse Serum (n=4) 94 86-104
Rat EDTA Plasma (n=4) 99 86-113
Rat Serum (n=4) 98 88-113
Urine (n=4) 91 85-109
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of Endothelin-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Endothelin-1 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Endothelin-1
Endothelin-1 (ET-1) is produced by many tissues including lung, kidney, brain, endocrine glands, and placenta. It is also expressed by several cell types including, mast cells, endothelial cells, epithelial and smooth muscle cells. The most prominent source is vascular endothelium. ET-1 is best known as a potent vasoconstrictor. Elevated blood ET-1 levels are associated with a variety of diseases, possibly as a function of a stress response.
    • Entrez Gene IDs
      1906 (Human); 13614 (Mouse); 24323 (Rat);
    • Alternate Names
      EDN1; endothelin 1; Endothelin-1; ET1; HDLCQ7; PPET1; preproendothelin-1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 150 µL Assay Diluent
    4.   Add 150 µL of Assay Diluent to each well.

    5. 75 µL Standard, Control, or Sample
    6.   Add 75 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 3 hours on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    16 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. HMGB1 contributes to glomerular endothelial cell injury in ANCA-associated vasculitis through enhancing endothelium-neutrophil interactions
      Authors: C Wang, DY Chang, M Chen, MH Zhao
      J. Cell. Mol. Med, 2017;0(0):.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. Peripheral venous congestion causes time- and dose-dependent release of endothelin-1 in humans
      Authors: J Lin, N Chudasama, Y Hayashi, C Hawk, SD Ramnauth, KY Wong, A Harxhi, D Onat, M Wakabayash, N Uriel, UP Jorde, TH LeJemtel, HN Sabbah, RT Demmer, PC Colombo
      Physiol Rep, 2017;5(6):.
      Species: Human
      Sample Type: Plasma
    3. The endothelin B receptor plays a crucial role for the adhesion of neutrophils to the endothelium in sickle cell disease
      Authors: B Koehl, P Nivoit, W El Nemer, O Lenoir, P Hermand, C Pereira, V Brousse, L Guyonnet, G Ghinatti, M Benkerrou, Y Colin, C Le Van Kim, PL Tharaux
      Haematologica, 2017;0(0):.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Preliminary study of hypoxia-related cardiovascular mediator-markers in patients with end-stage renal disease with and without diabetes and the effects of haemodialysis
      Authors: A Treweeke, J Hall, S Lambie, SJ Leslie, IL Megson, SM MacRury
      PLoS ONE, 2017;12(5):e0178171.
      Species: Human
      Sample Type: Plasma
    5. Cellular microbiology of Mycoplasma canis
      Authors: DL Michaels, JA Leibowitz, MT Azaiza, PK Shil, SM Shama, GF Kutish, SL Distelhors, MF Balish, MA May, DR Brown
      Infect Immun, 2016;0(0):.
      Species: Canine
      Sample Type: Cell Culture Supernates
    6. EDN1 Gene Variant is Associated with Neonatal Persistent Pulmonary Hypertension
      Sci Rep, 2016;6(0):29877.
      Species: Human
      Sample Type: Serum
    7. Citreoviridin Enhances Atherogenesis in Hypercholesterolemic ApoE-Deficient Mice via Upregulating Inflammation and Endothelial Dysfunction.
      Authors: Hou H, Yuan N, Guo Q, Sun T, Li C, Liu J, Li Q, Jiang B
      PLoS ONE, 2015;10(5):e0125956.
      Species: Mouse
      Sample Type: Serum
    8. Tumor necrosis factor-alpha-induced reduction of glomerular filtration rate in rats with fulminant hepatic failure.
      Authors: Wang J, Wang D, Wang H, Wang Z, Wen Y, Sun C, Zhao Y, Wu J, Liu P
      Lab Invest, 2014;94(7):740-51.
      Species: Rat
      Sample Type: Serum
    9. Hydroxycarbamide reduces eosinophil adhesion and degranulation in sickle cell anaemia patients.
      Authors: Pallis F, Conran N, Fertrin K, Olalla Saad S, Costa F, Franco-Penteado C
      Br J Haematol, 2014;164(2):286-95.
      Species: Human
      Sample Type: Plasma
    10. Endothelin A receptor antagonism enhances inhibitory effects of anti-ganglioside GD2 monoclonal antibody on invasiveness and viability of human osteosarcoma cells.
      Authors: Liu B, Wu Y, Zhou Y, Peng D
      PLoS ONE, 2014;9(4):e93576.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. TRPA1 controls inflammation and pruritogen responses in allergic contact dermatitis.
      Authors: Liu, Boyi, Escalera, Jasmine, Balakrishna, Shrilath, Fan, Lu, Caceres, Ana I, Robinson, Eve, Sui, Aiwei, McKay, M Craig, McAlexander, M Allen, Herrick, Christin, Jordt, Sven E
      FASEB J, 2013;27(9):3549-63.
      Species: Mouse
      Sample Type: Tissue Homogenates
    12. The mechanism through which octreotide inhibits hepatic stellate cell activity.
      Authors: Wang J, Wang L, Song G, Han B
      Mol Med Rep, 2013;7(5):1559-64.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Endothelin-1 induces endoplasmic reticulum stress by activating the PLC-IP(3) pathway: implications for placental pathophysiology in preeclampsia.
      Authors: Jain A, Olovsson M, Burton GJ, Yung HW
      Am. J. Pathol., 2012;180(6):2309-20.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in ApoE-deficient mice.
      Authors: Sang, Hui, Yuan, Na, Yao, Shutong, Li, Furong, Wang, Jiafu, Fang, Yongqi, Qin, Shucun
      Lipids Health Dis, 2012;11(0):166.
      Species: Mouse
      Sample Type: Serum
    15. Increased levels of inflammatory cytokines and endothelin-1 in alveolar macrophages from patients with chronic heart failure.
      Authors: Sikkeland LI, Dahl CP, Ueland T, Andreassen AK, Gude E, Edvardsen T, Holm T, Yndestad A, Gullestad L, Kongerud J, Aukrust P, ØIe E
      PLoS ONE, 2012;7(5):e36815.
      Species: Human
      Sample Type: Plasma
    16. The endothelin receptor antagonist bosentan improves peripheral endothelial function in patients with type 2 diabetes mellitus and microalbuminuria: a randomised trial.
      Authors: Rafnsson A, Bohm F, Settergren M, Gonon A, Brismar K, Pernow J
      Diabetologia, 2011;55(3):600-7.
      Species: Human
      Sample Type: Plasma
    Expand to show all 16 Citations
    ELISA Controls
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    Quantikine Immunoassay Control Set 907 for Endothelin-1

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    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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    Average Rating: 5 (Based on 2 reviews)

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     Endothelin-1 Quantikine ELISA Kit DET100
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      Anonymous 10/23/2017
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     Endothelin-1 Quantikine ELISA Kit DET100
    : Endothelin-1 Quantikine ELISA Kit [DET100]

    Summary

    Sample TestedPlasma

    Other Experimental Details

    Other Experimental DetailsPlasma was used from male mice.
     Endothelin-1 Quantikine ELISA Kit DET100
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      Anonymous 08/02/2017
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     Endothelin-1 Quantikine ELISA Kit DET100
    : Endothelin-1 Quantikine ELISA Kit [DET100]

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    Sample TestedSerum and Plasma

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