Endothelin-3 Antibody (2A6-2A4) - Azide and BSA Free

Novus Biologicals | Catalog # H00001908-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2A6-2A4

Format

Azide and BSA Free
View all Endothelin-3 Primary Antibodies »

Product Specifications

Immunogen

EDN3 (AAH08876, 1 a.a. ~ 238 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEPGLWLLFGLTVTSAAGFVPCSQSGDAGRRGVSQAPTAARSEGDCEETVAGPGEETVAGPGEGTVAPTALQGPSPGSPGQEQAAEGAPEHHRSRRCTCFTYKDKECVYYCHLDIIWINTPEQTVPYGLSNYRGSFRGKRSAGPLPGNLQLSHRPHLRCACVGRYDKACLHFCTQTLDVSSNSRTAEKTDKEEEGKVEVKDQQSKQALDLHHPKLMPGSGLALAPSTCPRCLFQEGAP

Specificity

EDN3 - endothelin 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Endothelin-3 Antibody (2A6-2A4) - Azide and BSA Free

Western Blot: Endothelin-3 Antibody (2A6-2A4) [H00001908-M01]

Western Blot: Endothelin-3 Antibody (2A6-2A4) [H00001908-M01]

Western Blot: Endothelin-3 Antibody (2A6-2A4) [H00001908-M01] - Analysis of EDN3 expression in transfected 293T cell line by EDN3 monoclonal antibody (M01), clone 2A6-2A4.Lane 1: EDN3 transfected lysate(25.5 KDa).Lane 2: Non-transfected lysate.
Western Blot: Endothelin-3 Antibody (2A6-2A4) [H00001908-M01]

Western Blot: Endothelin-3 Antibody (2A6-2A4) [H00001908-M01]

Western Blot: Endothelin-3 Antibody (2A6-2A4) [H00001908-M01] - EDN3 monoclonal antibody (M01), clone 2A6-2A4 Analysis of EDN3 expression in LNCaP.
Immunoprecipitation: Endothelin-3 Antibody (2A6-2A4) [H00001908-M01]

Immunoprecipitation: Endothelin-3 Antibody (2A6-2A4) [H00001908-M01]

Immunoprecipitation: Endothelin-3 Antibody (2A6-2A4) [H00001908-M01] - Analysis of EDN3 transfected lysate using anti-EDN3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EDN3 MaxPab rabbit polyclonal antibody.
ELISA: Endothelin-3 Antibody (2A6-2A4) [H00001908-M01]

ELISA: Endothelin-3 Antibody (2A6-2A4) [H00001908-M01]

ELISA: Endothelin-3 Antibody (2A6-2A4) [H00001908-M01] - Detection limit for recombinant GST tagged EDN3 is approximately 0.3ng/ml as a capture antibody.

Applications for Endothelin-3 Antibody (2A6-2A4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Endothelin-3

The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Four alternatively spliced transcript variants encoding three distinct isoforms have been observed.

Alternate Names

EDN3, Endothelin3

Entrez Gene IDs

1908 (Human)

Gene Symbol

EDN3

OMIM

131 (Human)

UniProt

AAH08876 (Human)

Additional Endothelin-3 Products

Product Documents for Endothelin-3 Antibody (2A6-2A4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Endothelin-3 Antibody (2A6-2A4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Endothelin-3 Antibody (2A6-2A4) - Azide and BSA Free

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