eNOS [p Ser116] Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-29664

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Bovine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all eNOS Primary Antibodies »

Product Specifications

Immunogen

Peptide corresponding to amino acids 106-122 of human eNOS/NOS III (Ser116).

Modification

p Ser116

Specificity

eNOS/NOS III phosphorylated on serine 116.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

132 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Relevant Entrez Gene Numbers: NM_000603

Scientific Data Images for eNOS [p Ser116] Antibody - BSA Free

Western Blot: eNOS [p Ser116] Antibody [NBP2-29664]

Western Blot: eNOS [p Ser116] Antibody [NBP2-29664]

Western Blot: eNOS [p Ser116] Antibody [NBP2-29664] - Untreated (lane 1) and lambda phosphatase-treated (lane 2) Fetal bovine heart endothelial cell lysate was probed with anti-phospho-eNOS (1:1000).

Applications for eNOS [p Ser116] Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.2M Tris-Glycine (pH 7.4), 0.15M NaCl, 0.1mM EDTA and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: eNOS

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.

Long Name

Endothelial Nitric Oxide Synthase

Alternate Names

NOS3

Gene Symbol

NOS3

UniProt

P29474

Additional eNOS Products

Product Documents for eNOS [p Ser116] Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for eNOS [p Ser116] Antibody - BSA Free

Relevant Entrez Gene Numbers: NM_000603

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Oxidative Stress
Redox Enzymes

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